gyrA mutations in ciprofloxacin-resistant Bartonella bacilliformis strains obtained in vitro

Michael F. Minnick, Zachary R. Wilson, Laura S. Smitherman, D. Scott Samuels

Research output: Contribution to journalArticlepeer-review

Abstract

We isolated and characterized mutants of Bartonella bacilliformis that are resistant to the fluoroquinolone antibiotic ciprofloxacin, which targets the A subunit of DNA gyrase. Mutants had single point mutations in the gyrA gene that changed either Asp-90 to Gly or Asp-95 to Asn and had 3- or 16-fold higher resistance, respectively, to ciprofloxacin than did wild-type B. bacilliformis. Asp-95 is homologous to Asp-87 of Escherichia coli GyrA and is a common residue mutated in fluoroquinolone-resistant strains of other bacteria. This is the first report of a mutation at an Asp-90 homologue, which corresponds to Asp-82 in E. coli GyrA.

Original languageEnglish
Pages (from-to)383-386
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume47
Issue number1
DOIs
StatePublished - Jan 1 2003

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