TY - JOUR
T1 - gyrA mutations in ciprofloxacin-resistant Bartonella bacilliformis strains obtained in vitro
AU - Minnick, Michael F.
AU - Wilson, Zachary R.
AU - Smitherman, Laura S.
AU - Samuels, D. Scott
PY - 2003/1/1
Y1 - 2003/1/1
N2 - We isolated and characterized mutants of Bartonella bacilliformis that are resistant to the fluoroquinolone antibiotic ciprofloxacin, which targets the A subunit of DNA gyrase. Mutants had single point mutations in the gyrA gene that changed either Asp-90 to Gly or Asp-95 to Asn and had 3- or 16-fold higher resistance, respectively, to ciprofloxacin than did wild-type B. bacilliformis. Asp-95 is homologous to Asp-87 of Escherichia coli GyrA and is a common residue mutated in fluoroquinolone-resistant strains of other bacteria. This is the first report of a mutation at an Asp-90 homologue, which corresponds to Asp-82 in E. coli GyrA.
AB - We isolated and characterized mutants of Bartonella bacilliformis that are resistant to the fluoroquinolone antibiotic ciprofloxacin, which targets the A subunit of DNA gyrase. Mutants had single point mutations in the gyrA gene that changed either Asp-90 to Gly or Asp-95 to Asn and had 3- or 16-fold higher resistance, respectively, to ciprofloxacin than did wild-type B. bacilliformis. Asp-95 is homologous to Asp-87 of Escherichia coli GyrA and is a common residue mutated in fluoroquinolone-resistant strains of other bacteria. This is the first report of a mutation at an Asp-90 homologue, which corresponds to Asp-82 in E. coli GyrA.
UR - http://www.scopus.com/inward/record.url?scp=0037224463&partnerID=8YFLogxK
U2 - 10.1128/AAC.47.1.383-386.2003
DO - 10.1128/AAC.47.1.383-386.2003
M3 - Article
C2 - 12499219
AN - SCOPUS:0037224463
SN - 0066-4804
VL - 47
SP - 383
EP - 386
JO - Antimicrobial Agents and Chemotherapy
JF - Antimicrobial Agents and Chemotherapy
IS - 1
ER -
