gyrB mutations in coumermycin A1-resistant Borrelia burgdorferi

D. S. Samuels; R. T. Marconi; Mun Huang Wai Mun Huang; C. F. Garon

doi:10.1128/jb.176.10.3072-3075.1994

gyrB mutations in coumermycin A₁-resistant Borrelia burgdorferi

D. S. Samuels, R. T. Marconi, Mun Huang Wai Mun Huang, C. F. Garon

Biological Sciences

National Institutes of Health

Research output: Contribution to journal › Comment/debate

53 Scopus citations

Abstract

We have isolated and characterized mutants of Borrelia burgdorferi that are resistant to the antibiotic coumermycin A₁, which targets the B subunit of DNA gyrase. Mutants had either 100- or 300-fold higher resistance to coumermycin A₁ than wild-type B. burgdorferi. In each case, a single point mutation in the gyrB gene converted Arg-133 to Gly or Ile. Mutations in the homologous Arg residue of Escherichia coli DNA gyrase are also associated with resistance to coumarin antimicrobial agents.

Original language	English
Pages (from-to)	3072-3075
Number of pages	4
Journal	Journal of Bacteriology
Volume	176
Issue number	10
DOIs	https://doi.org/10.1128/jb.176.10.3072-3075.1994
State	Published - 1994

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10.1128/jb.176.10.3072-3075.1994

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title = "gyrB mutations in coumermycin A1-resistant Borrelia burgdorferi",

abstract = "We have isolated and characterized mutants of Borrelia burgdorferi that are resistant to the antibiotic coumermycin A1, which targets the B subunit of DNA gyrase. Mutants had either 100- or 300-fold higher resistance to coumermycin A1 than wild-type B. burgdorferi. In each case, a single point mutation in the gyrB gene converted Arg-133 to Gly or Ile. Mutations in the homologous Arg residue of Escherichia coli DNA gyrase are also associated with resistance to coumarin antimicrobial agents.",

author = "Samuels, {D. S.} and Marconi, {R. T.} and {Wai Mun Huang}, {Mun Huang} and Garon, {C. F.}",

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AU - Samuels, D. S.

AU - Marconi, R. T.

AU - Wai Mun Huang, Mun Huang

AU - Garon, C. F.

PY - 1994

Y1 - 1994

N2 - We have isolated and characterized mutants of Borrelia burgdorferi that are resistant to the antibiotic coumermycin A1, which targets the B subunit of DNA gyrase. Mutants had either 100- or 300-fold higher resistance to coumermycin A1 than wild-type B. burgdorferi. In each case, a single point mutation in the gyrB gene converted Arg-133 to Gly or Ile. Mutations in the homologous Arg residue of Escherichia coli DNA gyrase are also associated with resistance to coumarin antimicrobial agents.

AB - We have isolated and characterized mutants of Borrelia burgdorferi that are resistant to the antibiotic coumermycin A1, which targets the B subunit of DNA gyrase. Mutants had either 100- or 300-fold higher resistance to coumermycin A1 than wild-type B. burgdorferi. In each case, a single point mutation in the gyrB gene converted Arg-133 to Gly or Ile. Mutations in the homologous Arg residue of Escherichia coli DNA gyrase are also associated with resistance to coumarin antimicrobial agents.

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U2 - 10.1128/jb.176.10.3072-3075.1994

DO - 10.1128/jb.176.10.3072-3075.1994

M3 - Comment/debate

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SP - 3072

EP - 3075

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 10

ER -

gyrB mutations in coumermycin A₁-resistant Borrelia burgdorferi

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