Abstract
High-throughput techniques based on restriction site-associated DNA sequencing (RADseq) are enabling the low-cost discovery and genotyping of thousands of genetic markers for any species, including non-model organisms, which is revolutionizing ecological, evolutionary and conservation genetics. Technical differences among these methods lead to important considerations for all steps of genomics studies, from the specific scientific questions that can be addressed, and the costs of library preparation and sequencing, to the types of bias and error inherent in the resulting data. In this Review, we provide a comprehensive discussion of RADseq methods to aid researchers in choosing among the many different approaches and avoiding erroneous scientific conclusions from RADseq data, a problem that has plagued other genetic marker types in the past.
| Original language | English |
|---|---|
| Pages (from-to) | 81-92 |
| Number of pages | 12 |
| Journal | Nature Reviews Genetics |
| Volume | 17 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 1 2016 |
Funding
The authors thank M. Gaither, E. Carroll, A. Moura, R. Bracewell and M. Jones for helpful discussions. K.R.A. was supported by the University of Idaho College of Natural Resources, USA. P.A.H. received support from US National Institutes of Health (NIH) grant P30 GM103324 and NSF grant 1316549. J.M.G. is supported by the Eunice Kennedy Shriver National Institute of Child Health and Human Development (R01HD73439) and the National Institute of General Medical Sciences (R01GM098536) of the National Institutes of Health. G.L. was supported by grants from US National Science Foundation (DEB-0742181 and DEB-1067613) and NASA-(NNX14AB84G).
| Funders | Funder number |
|---|---|
| 1316549, DEB-0742181, DEB-1067613 | |
| R01GM098536, P30GM103324 | |
| National Aeronautics and Space Administration | NNX14AB84G |
| R01HD73439 |