TY - JOUR
T1 - High-performance liquid chromatography study of the pharmacokinetics of various spin traps-for application to in vivo spin trapping
AU - Liu, Ke Jian
AU - Kotake, Yashige
AU - Lee, Margaret
AU - Miyake, Minoru
AU - Sugden, Kent
AU - Yu, Zhenqiang
AU - Swartz, Harold M.
N1 - Funding Information:
We are grateful to Dr. Edward G. Janzen for allowing us to use spin traps that were synthesized by his group at the University of Georgia, the University of Guelph, and the Oklahoma Medical Research Foundation. This research is supported in part by a grant from National Institutes of Health (R21 HL60623) and the Animal Gift Program of Charles River Laboratory. This research also used the facilities of the EPR Center for the Study of Viable Systems at Dartmouth that is supported by the National Institutes of Health Grant P41 RR11602. The authors thank Dr. G. M. Rosen for many helpful discussion and comments of the manuscript.
PY - 1999/7
Y1 - 1999/7
N2 - In vivo spin trapping is potentially a very useful tool to investigate the role of free radicals in physiologic processes and disease development. Unfortunately, knowledge on the stability and distribution of spin traps in living systems is limited. Therefore, in our study, we selected 11 acyclic and cyclic nitrone spin traps with diverse properties to determine their pharmacokinetics in mice. At varying times after intraperitoneal administration, we measured the concentration of the spin traps in the liver, heart, and blood. Our results showed that most spin traps were rapidly absorbed and were approximately evenly distributed throughout the mouse body. It was also found that most of the traps were relatively stable in vivo with more than half of the injected amount still available for spin trapping free radicals after an hour. Two of the 11 tested spin traps, however, decomposed after injection. These results indicate that for a successful in vivo spin trapping experiment, the stability of the spin trap is not of major concern, but the time course of distribution may be important.
AB - In vivo spin trapping is potentially a very useful tool to investigate the role of free radicals in physiologic processes and disease development. Unfortunately, knowledge on the stability and distribution of spin traps in living systems is limited. Therefore, in our study, we selected 11 acyclic and cyclic nitrone spin traps with diverse properties to determine their pharmacokinetics in mice. At varying times after intraperitoneal administration, we measured the concentration of the spin traps in the liver, heart, and blood. Our results showed that most spin traps were rapidly absorbed and were approximately evenly distributed throughout the mouse body. It was also found that most of the traps were relatively stable in vivo with more than half of the injected amount still available for spin trapping free radicals after an hour. Two of the 11 tested spin traps, however, decomposed after injection. These results indicate that for a successful in vivo spin trapping experiment, the stability of the spin trap is not of major concern, but the time course of distribution may be important.
KW - Electron paramagnetic resonance
KW - Free radicals
KW - High-performance liquid chromatography
KW - Pharmacokinetics
KW - Spin trapping
UR - http://www.scopus.com/inward/record.url?scp=0032782250&partnerID=8YFLogxK
U2 - 10.1016/S0891-5849(99)00042-8
DO - 10.1016/S0891-5849(99)00042-8
M3 - Article
C2 - 10443923
AN - SCOPUS:0032782250
SN - 0891-5849
VL - 27
SP - 82
EP - 89
JO - Free Radical Biology and Medicine
JF - Free Radical Biology and Medicine
IS - 1-2
ER -