High-performance liquid chromatography study of the pharmacokinetics of various spin traps-for application to in vivo spin trapping

Ke Jian Liu; Yashige Kotake; Margaret Lee; Minoru Miyake; Kent Sugden; Zhenqiang Yu; Harold M. Swartz

doi:10.1016/S0891-5849(99)00042-8

High-performance liquid chromatography study of the pharmacokinetics of various spin traps-for application to in vivo spin trapping

Ke Jian Liu
, Yashige Kotake
, Margaret Lee
, Minoru Miyake
, Kent Sugden
, Zhenqiang Yu
, Harold M. Swartz

Chemistry and Biochemistry

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

In vivo spin trapping is potentially a very useful tool to investigate the role of free radicals in physiologic processes and disease development. Unfortunately, knowledge on the stability and distribution of spin traps in living systems is limited. Therefore, in our study, we selected 11 acyclic and cyclic nitrone spin traps with diverse properties to determine their pharmacokinetics in mice. At varying times after intraperitoneal administration, we measured the concentration of the spin traps in the liver, heart, and blood. Our results showed that most spin traps were rapidly absorbed and were approximately evenly distributed throughout the mouse body. It was also found that most of the traps were relatively stable in vivo with more than half of the injected amount still available for spin trapping free radicals after an hour. Two of the 11 tested spin traps, however, decomposed after injection. These results indicate that for a successful in vivo spin trapping experiment, the stability of the spin trap is not of major concern, but the time course of distribution may be important.

Original language	English
Pages (from-to)	82-89
Number of pages	8
Journal	Free Radical Biology and Medicine
Volume	27
Issue number	1-2
DOIs	https://doi.org/10.1016/S0891-5849(99)00042-8
State	Published - Jul 1999

Funding

We are grateful to Dr. Edward G. Janzen for allowing us to use spin traps that were synthesized by his group at the University of Georgia, the University of Guelph, and the Oklahoma Medical Research Foundation. This research is supported in part by a grant from National Institutes of Health (R21 HL60623) and the Animal Gift Program of Charles River Laboratory. This research also used the facilities of the EPR Center for the Study of Viable Systems at Dartmouth that is supported by the National Institutes of Health Grant P41 RR11602. The authors thank Dr. G. M. Rosen for many helpful discussion and comments of the manuscript.

Funder number
P41 RR11602
R21 HL60623
P41RR011602

Keywords

Electron paramagnetic resonance
Free radicals
High-performance liquid chromatography
Pharmacokinetics
Spin trapping

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10.1016/S0891-5849(99)00042-8

Cite this

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title = "High-performance liquid chromatography study of the pharmacokinetics of various spin traps-for application to in vivo spin trapping",

abstract = "In vivo spin trapping is potentially a very useful tool to investigate the role of free radicals in physiologic processes and disease development. Unfortunately, knowledge on the stability and distribution of spin traps in living systems is limited. Therefore, in our study, we selected 11 acyclic and cyclic nitrone spin traps with diverse properties to determine their pharmacokinetics in mice. At varying times after intraperitoneal administration, we measured the concentration of the spin traps in the liver, heart, and blood. Our results showed that most spin traps were rapidly absorbed and were approximately evenly distributed throughout the mouse body. It was also found that most of the traps were relatively stable in vivo with more than half of the injected amount still available for spin trapping free radicals after an hour. Two of the 11 tested spin traps, however, decomposed after injection. These results indicate that for a successful in vivo spin trapping experiment, the stability of the spin trap is not of major concern, but the time course of distribution may be important.",

keywords = "Electron paramagnetic resonance, Free radicals, High-performance liquid chromatography, Pharmacokinetics, Spin trapping",

author = "Liu, \{Ke Jian\} and Yashige Kotake and Margaret Lee and Minoru Miyake and Kent Sugden and Zhenqiang Yu and Swartz, \{Harold M.\}",

note = "Funding Information: We are grateful to Dr. Edward G. Janzen for allowing us to use spin traps that were synthesized by his group at the University of Georgia, the University of Guelph, and the Oklahoma Medical Research Foundation. This research is supported in part by a grant from National Institutes of Health (R21 HL60623) and the Animal Gift Program of Charles River Laboratory. This research also used the facilities of the EPR Center for the Study of Viable Systems at Dartmouth that is supported by the National Institutes of Health Grant P41 RR11602. The authors thank Dr. G. M. Rosen for many helpful discussion and comments of the manuscript. ",

year = "1999",

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doi = "10.1016/S0891-5849(99)00042-8",

language = "English",

volume = "27",

pages = "82--89",

journal = "Free Radical Biology and Medicine",

issn = "0891-5849",

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TY - JOUR

T1 - High-performance liquid chromatography study of the pharmacokinetics of various spin traps-for application to in vivo spin trapping

AU - Liu, Ke Jian

AU - Kotake, Yashige

AU - Lee, Margaret

AU - Miyake, Minoru

AU - Sugden, Kent

AU - Yu, Zhenqiang

AU - Swartz, Harold M.

N1 - Funding Information: We are grateful to Dr. Edward G. Janzen for allowing us to use spin traps that were synthesized by his group at the University of Georgia, the University of Guelph, and the Oklahoma Medical Research Foundation. This research is supported in part by a grant from National Institutes of Health (R21 HL60623) and the Animal Gift Program of Charles River Laboratory. This research also used the facilities of the EPR Center for the Study of Viable Systems at Dartmouth that is supported by the National Institutes of Health Grant P41 RR11602. The authors thank Dr. G. M. Rosen for many helpful discussion and comments of the manuscript.

PY - 1999/7

Y1 - 1999/7

N2 - In vivo spin trapping is potentially a very useful tool to investigate the role of free radicals in physiologic processes and disease development. Unfortunately, knowledge on the stability and distribution of spin traps in living systems is limited. Therefore, in our study, we selected 11 acyclic and cyclic nitrone spin traps with diverse properties to determine their pharmacokinetics in mice. At varying times after intraperitoneal administration, we measured the concentration of the spin traps in the liver, heart, and blood. Our results showed that most spin traps were rapidly absorbed and were approximately evenly distributed throughout the mouse body. It was also found that most of the traps were relatively stable in vivo with more than half of the injected amount still available for spin trapping free radicals after an hour. Two of the 11 tested spin traps, however, decomposed after injection. These results indicate that for a successful in vivo spin trapping experiment, the stability of the spin trap is not of major concern, but the time course of distribution may be important.

AB - In vivo spin trapping is potentially a very useful tool to investigate the role of free radicals in physiologic processes and disease development. Unfortunately, knowledge on the stability and distribution of spin traps in living systems is limited. Therefore, in our study, we selected 11 acyclic and cyclic nitrone spin traps with diverse properties to determine their pharmacokinetics in mice. At varying times after intraperitoneal administration, we measured the concentration of the spin traps in the liver, heart, and blood. Our results showed that most spin traps were rapidly absorbed and were approximately evenly distributed throughout the mouse body. It was also found that most of the traps were relatively stable in vivo with more than half of the injected amount still available for spin trapping free radicals after an hour. Two of the 11 tested spin traps, however, decomposed after injection. These results indicate that for a successful in vivo spin trapping experiment, the stability of the spin trap is not of major concern, but the time course of distribution may be important.

KW - Electron paramagnetic resonance

KW - Free radicals

KW - High-performance liquid chromatography

KW - Pharmacokinetics

KW - Spin trapping

UR - https://www.scopus.com/pages/publications/0032782250

U2 - 10.1016/S0891-5849(99)00042-8

DO - 10.1016/S0891-5849(99)00042-8

M3 - Article

C2 - 10443923

AN - SCOPUS:0032782250

SN - 0891-5849

VL - 27

SP - 82

EP - 89

JO - Free Radical Biology and Medicine

JF - Free Radical Biology and Medicine

IS - 1-2

ER -

High-performance liquid chromatography study of the pharmacokinetics of various spin traps-for application to in vivo spin trapping

Abstract

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Keywords

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