"HOOF-Print" genotyping and haplotype inference discriminates among Brucella spp. isolates from a small spatial scale

Cláudia Almendra, Teresa Luísa Silva, Albano Beja-Pereira, Ana Cristina Ferreira, Lénia Ferrão-Beck, Maria Inácia Corrêa de Sá, Betsy J. Bricker, Gordon Luikart

Research output: Contribution to journalArticlepeer-review

Abstract

We demonstrate that the "HOOF-Print" assay provides high power to discriminate among Brucella isolates collected on a small spatial scale (within Portugal). Additionally, we illustrate how haplotype identification using non-random association among markers allows resolution of B. melitensis biovars (1 and 3). We recommend that future studies use haplotype identification when analyzing multilocus population genetic data to help discriminate among microbial isolates such as Brucella.

Original languageEnglish
Pages (from-to)104-107
Number of pages4
JournalInfection, Genetics and Evolution
Volume9
Issue number1
DOIs
StatePublished - Jan 2009

Keywords

  • Biovar discrimination
  • Brucella spp.
  • Gametic disequilibrium
  • Genotyping analysis
  • HOOF-print assay
  • Population genetics

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