"HOOF-Print" genotyping and haplotype inference discriminates among Brucella spp. isolates from a small spatial scale

Cláudia Almendra; Teresa Luísa Silva; Albano Beja-Pereira; Ana Cristina Ferreira; Lénia Ferrão-Beck; Maria Inácia Corrêa de Sá; Betsy J. Bricker; Gordon Luikart

doi:10.1016/j.meegid.2008.10.007

"HOOF-Print" genotyping and haplotype inference discriminates among Brucella spp. isolates from a small spatial scale

Cláudia Almendra
, Teresa Luísa Silva
, Albano Beja-Pereira
, Ana Cristina Ferreira
, Lénia Ferrão-Beck
, Maria Inácia Corrêa de Sá
, Betsy J. Bricker
, Gordon Luikart

Flathead Lake Biological Station

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

We demonstrate that the "HOOF-Print" assay provides high power to discriminate among Brucella isolates collected on a small spatial scale (within Portugal). Additionally, we illustrate how haplotype identification using non-random association among markers allows resolution of B. melitensis biovars (1 and 3). We recommend that future studies use haplotype identification when analyzing multilocus population genetic data to help discriminate among microbial isolates such as Brucella.

Original language	English
Pages (from-to)	104-107
Number of pages	4
Journal	Infection, Genetics and Evolution
Volume	9
Issue number	1
DOIs	https://doi.org/10.1016/j.meegid.2008.10.007
State	Published - Jan 2009

Funding

CA, ABP and TLS are supported by national grants from Science and Technology Foundation (FCT; SFRH/BD30737/2006; SFRH/BPD/17822/2004) and GL supported by the Luso-American Foundation for Development (FLAD), CIBIO-UP and the University of Montana. This work was partially financed by a project grant from FCT POCI/CVT/56758/2004 and PTDC/CVT/69438/2006).

Funder number
POCI/CVT/56758/2004, PTDC/CVT/69438/2006
SFRH/BD30737/2006, SFRH/BPD/17822/2004

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Biovar discrimination
Brucella spp.
Gametic disequilibrium
Genotyping analysis
HOOF-print assay
Population genetics

Access to Document

10.1016/j.meegid.2008.10.007

Cite this

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title = "{"}HOOF-Print{"} genotyping and haplotype inference discriminates among Brucella spp. isolates from a small spatial scale",

abstract = "We demonstrate that the {"}HOOF-Print{"} assay provides high power to discriminate among Brucella isolates collected on a small spatial scale (within Portugal). Additionally, we illustrate how haplotype identification using non-random association among markers allows resolution of B. melitensis biovars (1 and 3). We recommend that future studies use haplotype identification when analyzing multilocus population genetic data to help discriminate among microbial isolates such as Brucella.",

keywords = "Biovar discrimination, Brucella spp., Gametic disequilibrium, Genotyping analysis, HOOF-print assay, Population genetics",

author = "Cl{\'a}udia Almendra and Silva, \{Teresa Lu{\'i}sa\} and Albano Beja-Pereira and Ferreira, \{Ana Cristina\} and L{\'e}nia Ferr{\~a}o-Beck and S{\'a}, \{Maria In{\'a}cia Corr{\^e}a de\} and Bricker, \{Betsy J.\} and Gordon Luikart",

note = "Funding Information: CA, ABP and TLS are supported by national grants from Science and Technology Foundation (FCT; SFRH/BD30737/2006; SFRH/BPD/17822/2004) and GL supported by the Luso-American Foundation for Development (FLAD), CIBIO-UP and the University of Montana. This work was partially financed by a project grant from FCT POCI/CVT/56758/2004 and PTDC/CVT/69438/2006). ",

year = "2009",

month = jan,

doi = "10.1016/j.meegid.2008.10.007",

language = "English",

volume = "9",

pages = "104--107",

journal = "Infection, Genetics and Evolution",

issn = "1567-1348",

publisher = "Elsevier B.V.",

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T1 - "HOOF-Print" genotyping and haplotype inference discriminates among Brucella spp. isolates from a small spatial scale

AU - Almendra, Cláudia

AU - Silva, Teresa Luísa

AU - Beja-Pereira, Albano

AU - Ferreira, Ana Cristina

AU - Ferrão-Beck, Lénia

AU - Sá, Maria Inácia Corrêa de

AU - Bricker, Betsy J.

AU - Luikart, Gordon

N1 - Funding Information: CA, ABP and TLS are supported by national grants from Science and Technology Foundation (FCT; SFRH/BD30737/2006; SFRH/BPD/17822/2004) and GL supported by the Luso-American Foundation for Development (FLAD), CIBIO-UP and the University of Montana. This work was partially financed by a project grant from FCT POCI/CVT/56758/2004 and PTDC/CVT/69438/2006).

PY - 2009/1

Y1 - 2009/1

N2 - We demonstrate that the "HOOF-Print" assay provides high power to discriminate among Brucella isolates collected on a small spatial scale (within Portugal). Additionally, we illustrate how haplotype identification using non-random association among markers allows resolution of B. melitensis biovars (1 and 3). We recommend that future studies use haplotype identification when analyzing multilocus population genetic data to help discriminate among microbial isolates such as Brucella.

AB - We demonstrate that the "HOOF-Print" assay provides high power to discriminate among Brucella isolates collected on a small spatial scale (within Portugal). Additionally, we illustrate how haplotype identification using non-random association among markers allows resolution of B. melitensis biovars (1 and 3). We recommend that future studies use haplotype identification when analyzing multilocus population genetic data to help discriminate among microbial isolates such as Brucella.

KW - Biovar discrimination

KW - Brucella spp.

KW - Gametic disequilibrium

KW - Genotyping analysis

KW - HOOF-print assay

KW - Population genetics

UR - https://www.scopus.com/pages/publications/57549097069

U2 - 10.1016/j.meegid.2008.10.007

DO - 10.1016/j.meegid.2008.10.007

M3 - Article

C2 - 19022402

AN - SCOPUS:57549097069

SN - 1567-1348

VL - 9

SP - 104

EP - 107

JO - Infection, Genetics and Evolution

JF - Infection, Genetics and Evolution

IS - 1

ER -

"HOOF-Print" genotyping and haplotype inference discriminates among Brucella spp. isolates from a small spatial scale

Abstract

Funding

UN SDGs

Keywords

Access to Document

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Cite this