Identification and interpretation of complexity in sedimentation velocity boundaries

Borries Demeler; Hashim Saber; Jeffrey C. Hansen

doi:10.1016/S0006-3495(97)78680-6

Identification and interpretation of complexity in sedimentation velocity boundaries

Borries Demeler, Hashim Saber, Jeffrey C. Hansen

Chemistry and Biochemistry

Research output: Contribution to journal › Article › peer-review

143 Scopus citations

Abstract

Synthetic sedimentation velocity boundaries were generated using finite- element solutions to the original and modified forms of the Lamm equation. Situations modeled included ideal single- and multicomponent samples, concentration-dependent samples, noninteracting multicomponent samples, and reversibly self-associating samples. Synthetic boundaries subsequently were analyzed using the method of van Halde and Weischet, and results were compared against known input parameters. Results indicate that this analytical method provides rigorous diagnostics for virtually every type of sample complexity encountered experimentally. Accordingly, both the power and utility of sedimentation velocity experiments have been significantly expanded.

Original language	English
Pages (from-to)	397-407
Number of pages	11
Journal	Biophysical Journal
Volume	72
Issue number	1
DOIs	https://doi.org/10.1016/S0006-3495(97)78680-6
State	Published - Jan 1997

Funding

This work was supported by National Institutes of Health grant GM45916 (to JCH) and by the University of Texas Health Science Center at San Antonio Department of Biochemistry.

Funder number
R29GM045916

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10.1016/S0006-3495(97)78680-6

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title = "Identification and interpretation of complexity in sedimentation velocity boundaries",

abstract = "Synthetic sedimentation velocity boundaries were generated using finite- element solutions to the original and modified forms of the Lamm equation. Situations modeled included ideal single- and multicomponent samples, concentration-dependent samples, noninteracting multicomponent samples, and reversibly self-associating samples. Synthetic boundaries subsequently were analyzed using the method of van Halde and Weischet, and results were compared against known input parameters. Results indicate that this analytical method provides rigorous diagnostics for virtually every type of sample complexity encountered experimentally. Accordingly, both the power and utility of sedimentation velocity experiments have been significantly expanded.",

author = "Borries Demeler and Hashim Saber and Hansen, \{Jeffrey C.\}",

note = "Funding Information: This work was supported by National Institutes of Health grant GM45916 (to JCH) and by the University of Texas Health Science Center at San Antonio Department of Biochemistry.",

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AU - Saber, Hashim

AU - Hansen, Jeffrey C.

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PY - 1997/1

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N2 - Synthetic sedimentation velocity boundaries were generated using finite- element solutions to the original and modified forms of the Lamm equation. Situations modeled included ideal single- and multicomponent samples, concentration-dependent samples, noninteracting multicomponent samples, and reversibly self-associating samples. Synthetic boundaries subsequently were analyzed using the method of van Halde and Weischet, and results were compared against known input parameters. Results indicate that this analytical method provides rigorous diagnostics for virtually every type of sample complexity encountered experimentally. Accordingly, both the power and utility of sedimentation velocity experiments have been significantly expanded.

AB - Synthetic sedimentation velocity boundaries were generated using finite- element solutions to the original and modified forms of the Lamm equation. Situations modeled included ideal single- and multicomponent samples, concentration-dependent samples, noninteracting multicomponent samples, and reversibly self-associating samples. Synthetic boundaries subsequently were analyzed using the method of van Halde and Weischet, and results were compared against known input parameters. Results indicate that this analytical method provides rigorous diagnostics for virtually every type of sample complexity encountered experimentally. Accordingly, both the power and utility of sedimentation velocity experiments have been significantly expanded.

UR - https://www.scopus.com/pages/publications/0031020113

U2 - 10.1016/S0006-3495(97)78680-6

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VL - 72

SP - 397

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JO - Biophysical Journal

JF - Biophysical Journal

IS - 1

ER -

Identification and interpretation of complexity in sedimentation velocity boundaries

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