Identification of Bartonella using PCR; genus- and species-specific primer sets

Michael F. Minnick; Kent D. Barbian

doi:10.1016/S0167-7012(97)00083-3

Identification of Bartonella using PCR; genus- and species-specific primer sets

Michael F. Minnick
, Kent D. Barbian

Division of Biological and Biomedical Sciences

University of Montana

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Four Bartonella species cause a variety of emerging diseases in humans that can be difficult to diagnose. A PCR-based identification system using amplimers designed from internal portions of the 16S-23S intergenic spacer (ITS) region is described. A genus-specific primer set can distinguish Bartonella species from Escherichia coli or closely-related α-Proteobacteria including Brucella abortus. Agrobacterium tumefaciens and Rhizobium meliloti. Species-specific primer sets for bartonellae that cause human disease (B. bacilliformis, B. elizabethae, B. henselae and B. quintana) produce single, unique amplicons from their respective target and do not react with DNA from other Bartonella species, close bacterial relatives, or E. coli. The described system streamlines current PCR-based identification of pathogenic bartonellae by generating genus- or species-specific amplicons, obviating the need for subsequent RFLP or sequence analysis of the PCR product.

Original language	English
Pages (from-to)	51-57
Number of pages	7
Journal	Journal of Microbiological Methods
Volume	31
Issue number	1-2
DOIs	https://doi.org/10.1016/S0167-7012(97)00083-3
State	Published - Dec 1 1997

Funding

We thank Drs. Russ Regnery, Todd Steck and Robert Tesh for the generous gifts of B. henselae, A. tumefaciens and B. bacilliformis (LA6.3), respectively. This work was supported in part by Public Health Service grant AI34050.

Funder number
AI34050

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Bartonella
Identification
PCR
Rapid method

Access to Document

10.1016/S0167-7012(97)00083-3

Cite this

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title = "Identification of Bartonella using PCR; genus- and species-specific primer sets",

abstract = "Four Bartonella species cause a variety of emerging diseases in humans that can be difficult to diagnose. A PCR-based identification system using amplimers designed from internal portions of the 16S-23S intergenic spacer (ITS) region is described. A genus-specific primer set can distinguish Bartonella species from Escherichia coli or closely-related α-Proteobacteria including Brucella abortus. Agrobacterium tumefaciens and Rhizobium meliloti. Species-specific primer sets for bartonellae that cause human disease (B. bacilliformis, B. elizabethae, B. henselae and B. quintana) produce single, unique amplicons from their respective target and do not react with DNA from other Bartonella species, close bacterial relatives, or E. coli. The described system streamlines current PCR-based identification of pathogenic bartonellae by generating genus- or species-specific amplicons, obviating the need for subsequent RFLP or sequence analysis of the PCR product.",

keywords = "Bartonella, Identification, PCR, Rapid method",

author = "Minnick, \{Michael F.\} and Barbian, \{Kent D.\}",

note = "Funding Information: We thank Drs. Russ Regnery, Todd Steck and Robert Tesh for the generous gifts of B. henselae, A. tumefaciens and B. bacilliformis (LA6.3), respectively. This work was supported in part by Public Health Service grant AI34050.",

year = "1997",

month = dec,

day = "1",

doi = "10.1016/S0167-7012(97)00083-3",

language = "English",

volume = "31",

pages = "51--57",

journal = "Journal of Microbiological Methods",

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T1 - Identification of Bartonella using PCR; genus- and species-specific primer sets

AU - Minnick, Michael F.

AU - Barbian, Kent D.

N1 - Funding Information: We thank Drs. Russ Regnery, Todd Steck and Robert Tesh for the generous gifts of B. henselae, A. tumefaciens and B. bacilliformis (LA6.3), respectively. This work was supported in part by Public Health Service grant AI34050.

PY - 1997/12/1

Y1 - 1997/12/1

N2 - Four Bartonella species cause a variety of emerging diseases in humans that can be difficult to diagnose. A PCR-based identification system using amplimers designed from internal portions of the 16S-23S intergenic spacer (ITS) region is described. A genus-specific primer set can distinguish Bartonella species from Escherichia coli or closely-related α-Proteobacteria including Brucella abortus. Agrobacterium tumefaciens and Rhizobium meliloti. Species-specific primer sets for bartonellae that cause human disease (B. bacilliformis, B. elizabethae, B. henselae and B. quintana) produce single, unique amplicons from their respective target and do not react with DNA from other Bartonella species, close bacterial relatives, or E. coli. The described system streamlines current PCR-based identification of pathogenic bartonellae by generating genus- or species-specific amplicons, obviating the need for subsequent RFLP or sequence analysis of the PCR product.

AB - Four Bartonella species cause a variety of emerging diseases in humans that can be difficult to diagnose. A PCR-based identification system using amplimers designed from internal portions of the 16S-23S intergenic spacer (ITS) region is described. A genus-specific primer set can distinguish Bartonella species from Escherichia coli or closely-related α-Proteobacteria including Brucella abortus. Agrobacterium tumefaciens and Rhizobium meliloti. Species-specific primer sets for bartonellae that cause human disease (B. bacilliformis, B. elizabethae, B. henselae and B. quintana) produce single, unique amplicons from their respective target and do not react with DNA from other Bartonella species, close bacterial relatives, or E. coli. The described system streamlines current PCR-based identification of pathogenic bartonellae by generating genus- or species-specific amplicons, obviating the need for subsequent RFLP or sequence analysis of the PCR product.

KW - Bartonella

KW - Identification

KW - PCR

KW - Rapid method

UR - https://www.scopus.com/pages/publications/0031444584

U2 - 10.1016/S0167-7012(97)00083-3

DO - 10.1016/S0167-7012(97)00083-3

M3 - Article

AN - SCOPUS:0031444584

SN - 0167-7012

VL - 31

SP - 51

EP - 57

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 1-2

ER -

Identification of Bartonella using PCR; genus- and species-specific primer sets

Abstract

Funding

UN SDGs

Keywords

Access to Document

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