In situ detection of ribonucleoprotein complex assembly in the C. Elegans germline using proximity ligation assay

Nicholas J. Day, Xiaobo Wang, Ekaterina Voronina

Research output: Contribution to journalArticlepeer-review

Abstract

Understanding when and where protein-protein interactions (PPIs) occur is critical to understanding protein function in the cell and how broader processes such as development are affected. The Caenorhabditis elegans germline is a great model system for studying PPIs that are related to the regulation of stem cells, meiosis, and development. There are a variety of well-developed techniques that allow proteins of interest to be tagged for recognition by standard antibodies, making this system advantageous for proximity ligation assay (PLA) reactions. As a result, the PLA is able to show where PPIs occur in a spatial and temporal manner in germlines more effectively than alternative approaches. Described here is a protocol for the application and quantification of this technology to probe PPIs in the C. elegans germline.

Original languageEnglish
Article numbere60982
JournalJournal of Visualized Experiments
Volume2020
Issue number159
DOIs
StatePublished - May 2020

Keywords

  • C. Elegans
  • Developmental Biology
  • Germline
  • Issue 159
  • LC8
  • PLA
  • Proximity ligation assay
  • RNA-binding protein

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