Inhibition of topoisomerase I by antibodies in sera from scleroderma patients

D. Scott Samuels, Takeshi Tojo, Mitsuo Homma, Nobuyoshi Shimizu

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Purified type I topoisomerase from calf thymus as well as nuclear and cytoplasmic extracts from EGF-stimulated human and mouse fibroblasts in cell culture efficiently convert supercoiled plasmid DNA to the relaxed form. The purified IgG fraction from the sera of Japanese patients with the rheumatic disease scleroderma were shown to inhibit this relaxation activity. Thus, these patients likely produce autoantibodies to topoisomerase I. In addition, the human, bovine and murine enzymes share antigenic determinants recognized by the antisera.

Original languageEnglish
Pages (from-to)231-234
Number of pages4
JournalFEBS Letters
Issue number2
StatePublished - Dec 15 1986


  • Epidermal growth factor
  • Scleroderma antiserum
  • Topoisomerase I


Dive into the research topics of 'Inhibition of topoisomerase I by antibodies in sera from scleroderma patients'. Together they form a unique fingerprint.

Cite this