Inhibition of topoisomerase I by antibodies in sera from scleroderma patients

D. Scott Samuels; Takeshi Tojo; Mitsuo Homma; Nobuyoshi Shimizu

doi:10.1016/0014-5793(86)81117-6

Inhibition of topoisomerase I by antibodies in sera from scleroderma patients

D. Scott Samuels, Takeshi Tojo, Mitsuo Homma, Nobuyoshi Shimizu

Biological Sciences

Keio University

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Purified type I topoisomerase from calf thymus as well as nuclear and cytoplasmic extracts from EGF-stimulated human and mouse fibroblasts in cell culture efficiently convert supercoiled plasmid DNA to the relaxed form. The purified IgG fraction from the sera of Japanese patients with the rheumatic disease scleroderma were shown to inhibit this relaxation activity. Thus, these patients likely produce autoantibodies to topoisomerase I. In addition, the human, bovine and murine enzymes share antigenic determinants recognized by the antisera.

Original language	English
Pages (from-to)	231-234
Number of pages	4
Journal	FEBS Letters
Volume	209
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(86)81117-6
State	Published - Dec 15 1986

Keywords

Epidermal growth factor
Scleroderma antiserum
Topoisomerase I

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0014-5793(86)81117-6

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title = "Inhibition of topoisomerase I by antibodies in sera from scleroderma patients",

abstract = "Purified type I topoisomerase from calf thymus as well as nuclear and cytoplasmic extracts from EGF-stimulated human and mouse fibroblasts in cell culture efficiently convert supercoiled plasmid DNA to the relaxed form. The purified IgG fraction from the sera of Japanese patients with the rheumatic disease scleroderma were shown to inhibit this relaxation activity. Thus, these patients likely produce autoantibodies to topoisomerase I. In addition, the human, bovine and murine enzymes share antigenic determinants recognized by the antisera.",

keywords = "Epidermal growth factor, Scleroderma antiserum, Topoisomerase I",

author = "{Scott Samuels}, D. and Takeshi Tojo and Mitsuo Homma and Nobuyoshi Shimizu",

note = "Funding Information: This work was supportedb y a grant from the NIH and a Grant-in-Aid from the Ministry of Education, Sciencea nd Culture, Japan. We thank Dr Yoshiko Shimizu for advicea nd MS TelsaM it-telmeierf or preparationo f supercoileds ubstrate DNA.",

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doi = "10.1016/0014-5793(86)81117-6",

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T1 - Inhibition of topoisomerase I by antibodies in sera from scleroderma patients

AU - Scott Samuels, D.

AU - Tojo, Takeshi

AU - Homma, Mitsuo

AU - Shimizu, Nobuyoshi

N1 - Funding Information: This work was supportedb y a grant from the NIH and a Grant-in-Aid from the Ministry of Education, Sciencea nd Culture, Japan. We thank Dr Yoshiko Shimizu for advicea nd MS TelsaM it-telmeierf or preparationo f supercoileds ubstrate DNA.

PY - 1986/12/15

Y1 - 1986/12/15

N2 - Purified type I topoisomerase from calf thymus as well as nuclear and cytoplasmic extracts from EGF-stimulated human and mouse fibroblasts in cell culture efficiently convert supercoiled plasmid DNA to the relaxed form. The purified IgG fraction from the sera of Japanese patients with the rheumatic disease scleroderma were shown to inhibit this relaxation activity. Thus, these patients likely produce autoantibodies to topoisomerase I. In addition, the human, bovine and murine enzymes share antigenic determinants recognized by the antisera.

AB - Purified type I topoisomerase from calf thymus as well as nuclear and cytoplasmic extracts from EGF-stimulated human and mouse fibroblasts in cell culture efficiently convert supercoiled plasmid DNA to the relaxed form. The purified IgG fraction from the sera of Japanese patients with the rheumatic disease scleroderma were shown to inhibit this relaxation activity. Thus, these patients likely produce autoantibodies to topoisomerase I. In addition, the human, bovine and murine enzymes share antigenic determinants recognized by the antisera.

KW - Epidermal growth factor

KW - Scleroderma antiserum

KW - Topoisomerase I

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JF - FEBS Letters

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ER -

Inhibition of topoisomerase I by antibodies in sera from scleroderma patients

Abstract

Keywords

UN SDGs

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