Integration host factor interactions with Neisseria gene sequences: Correlation between predicted binding sites and in vitro binding of Neisseria-derived IHF protein

S. A. Hill, D. S. Samuels, C. Nielsen, S. W. Knight, F. Pagotto, J. A.R. Dillon

Research output: Contribution to journalArticlepeer-review

Abstract

Putative integration host factor (IHF) binding sites are frequently being identified in Neisseria gene sequences on the basis of similarity to a degenerate Escherichia coli-derived consensus binding sequence. In this report, three different Neisseria genetic systems that contain predicted IHF binding sites were assessed for IHF binding through gel retardation analysis. The results show a positive correlation between the identification of a predicted Neisseria IHF binding site and in vitro binding of Neisseria-derived IHF protein.

Original languageEnglish
Pages (from-to)153-158
Number of pages6
JournalMolecular and Cellular Probes
Volume16
Issue number2
DOIs
StatePublished - 2002

Keywords

  • Gel retardation assays
  • Integration host factor
  • Neisseria

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