TY - JOUR
T1 - Intra-city differences in cardiac expression of inflammatory genes and inflammasomes in young urbanites
T2 - A pilot study
AU - Villarreal-Calderon, Rodolfo
AU - Dale, Gary
AU - Delgado-Chávez, Ricardo
AU - Torres-Jardón, Ricardo
AU - Zhu, Hongtu
AU - Herritt, Lou
AU - Gónzalez-Maciel, Angelica
AU - Reynoso-Robles, Rafael
AU - Yuan, Ying
AU - Wang, Jiaping
AU - Solorio-López, Edelmira
AU - Medina-Cortina, Humberto
AU - Calderón-Garcidueñas, Lilian
PY - 2012/7
Y1 - 2012/7
N2 - Southwest Mexico City (SWMC) air pollution is characterized by high concentrations of ozone and particulate matter < 10 μm (PM 10) containing lipopolysaccharides while in the North PM 2.5 is high. These intra-city differences are likely accounting for higher CD14 and IL-1β in SWMC v NMC mice myocardial expression. This pilot study was designed to investigate whether similar intra-city differences exist in the levels of myocardial inflammatory genes in young people. Inflammatory mediator genes and inflammasome arrays were measured in right and left autopsy ventricles of 6 southwest/15 north (18.5 ± 2.6 years) MC residents after fatal sudden accidental deaths. There was a significant S v N right ventricle up-regulation of IL-1β (p=0.008), TNF-α (p=0.001), IL-10 (p=0.001), and CD14 (p=0.002), and a left ventricle difference in TNF-α (p=0.007), and IL-10 (p=0.02). SW right ventricles had significant up-regulation of NLRC1, NLRP3 and of 29/84 inflammasome genes, including NOD factors and caspases. There was significant degranulation of mast cells both in myocardium and epicardial nerve fibers. Differential expression of key inflammatory myocardial genes and inflammasomes are influenced by the location of residence. Myocardial inflammation and inflammasome activation in young hearts is a plausible pathway of heart injury in urbanites and adverse effects on the cardiovascular system are expected.
AB - Southwest Mexico City (SWMC) air pollution is characterized by high concentrations of ozone and particulate matter < 10 μm (PM 10) containing lipopolysaccharides while in the North PM 2.5 is high. These intra-city differences are likely accounting for higher CD14 and IL-1β in SWMC v NMC mice myocardial expression. This pilot study was designed to investigate whether similar intra-city differences exist in the levels of myocardial inflammatory genes in young people. Inflammatory mediator genes and inflammasome arrays were measured in right and left autopsy ventricles of 6 southwest/15 north (18.5 ± 2.6 years) MC residents after fatal sudden accidental deaths. There was a significant S v N right ventricle up-regulation of IL-1β (p=0.008), TNF-α (p=0.001), IL-10 (p=0.001), and CD14 (p=0.002), and a left ventricle difference in TNF-α (p=0.007), and IL-10 (p=0.02). SW right ventricles had significant up-regulation of NLRC1, NLRP3 and of 29/84 inflammasome genes, including NOD factors and caspases. There was significant degranulation of mast cells both in myocardium and epicardial nerve fibers. Differential expression of key inflammatory myocardial genes and inflammasomes are influenced by the location of residence. Myocardial inflammation and inflammasome activation in young hearts is a plausible pathway of heart injury in urbanites and adverse effects on the cardiovascular system are expected.
KW - Cardiovascular risk factor
KW - Children
KW - Inflammasomes
KW - Mast cells
KW - Myocardial inflammation
KW - Particulate matter
KW - Urban pollution
UR - http://www.scopus.com/inward/record.url?scp=84863835727&partnerID=8YFLogxK
U2 - 10.1293/tox.25.163
DO - 10.1293/tox.25.163
M3 - Article
AN - SCOPUS:84863835727
SN - 0914-9198
VL - 25
SP - 163
EP - 173
JO - Journal of Toxicologic Pathology
JF - Journal of Toxicologic Pathology
IS - 2
ER -
