Isolation and identification of innate lymphoid cells (ILCs) for immunotoxicity testing

Elia D. Tait Wojno, Celine A. Beamer

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Innate lymphoid cells (ILCs) comprise a family of innate immune cells that orchestrate mucosal immune responses: initiating, sustaining, and even curbing immune responses. ILCs are relatively rare (≤1% of lymphocytes in mucosal tissues), lack classical cell-surface markers, and can be divided into three subsets (type 1-3 ILCs) based on differences in cytokine production, phenotype, and developmental pathway. Because ILCs can only be identified by combinations of cell-surface markers and cytokine production, multicolor flow cytometry is the most reliable method to purify, characterize, and assess the functionality of ILCs. Here, we describe the methods for cell preparation, flow cytometric analysis, and purification of murine ILCs from the lung.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages353-370
Number of pages18
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1803
ISSN (Print)1064-3745

Keywords

  • Flow cytometry
  • ILC2
  • ILC3
  • Innate lymphoid cells
  • Mouse
  • Respiratory tract

Fingerprint

Dive into the research topics of 'Isolation and identification of innate lymphoid cells (ILCs) for immunotoxicity testing'. Together they form a unique fingerprint.

Cite this