Leukotriene B4 stimulation of phosphatidylinositol turnover in macrophages and inhibition by pertussis toxin

Andrij Holian

doi:10.1016/0014-5793(86)80562-2

Leukotriene B₄ stimulation of phosphatidylinositol turnover in macrophages and inhibition by pertussis toxin

Andrij Holian

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Abstract

The influence of leukotriene B₄ (LTB₄) on phosphatidylinositol (PI) cycle activity was investigated in the guinea pig alveolar macrophage. In contrast to the observation reported in leukocytes [(1984) Proc. Natl. Acad. Sci. USA 81, 5966-5969], LTB₄ was found to stimulate PI cycle activity in the macrophage (transient decrease in phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 4-phosphate, transient elevation of 1,2-diacylglycerol and elevation of phosphatidic acid) similar to the stimulation of the PI cycle by the chemotactic formyi peptides. Preincubation of macrophages with 1 μ/ml of pertussis toxin, for 1 h at 37°C, blocked LTB₄ and formyl peptide stimulated O₂^- production and PI cycle activity. Therefore, both stimulants are proposed to act through the same coupling protein to activate phospholipase C in the early stages of macrophage activation.

Original language	English
Pages (from-to)	15-19
Number of pages	5
Journal	FEBS Letters
Volume	201
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(86)80562-2
State	Published - May 26 1986

Keywords

Formyl peptide
Leukotriene B
Macrophage
Pertussis toxin
Phosphatidylinositol

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10.1016/0014-5793(86)80562-2

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title = "Leukotriene B4 stimulation of phosphatidylinositol turnover in macrophages and inhibition by pertussis toxin",

abstract = "The influence of leukotriene B4 (LTB4) on phosphatidylinositol (PI) cycle activity was investigated in the guinea pig alveolar macrophage. In contrast to the observation reported in leukocytes [(1984) Proc. Natl. Acad. Sci. USA 81, 5966-5969], LTB4 was found to stimulate PI cycle activity in the macrophage (transient decrease in phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 4-phosphate, transient elevation of 1,2-diacylglycerol and elevation of phosphatidic acid) similar to the stimulation of the PI cycle by the chemotactic formyi peptides. Preincubation of macrophages with 1 μ/ml of pertussis toxin, for 1 h at 37°C, blocked LTB4 and formyl peptide stimulated O2- production and PI cycle activity. Therefore, both stimulants are proposed to act through the same coupling protein to activate phospholipase C in the early stages of macrophage activation.",

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AU - Holian, Andrij

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N2 - The influence of leukotriene B4 (LTB4) on phosphatidylinositol (PI) cycle activity was investigated in the guinea pig alveolar macrophage. In contrast to the observation reported in leukocytes [(1984) Proc. Natl. Acad. Sci. USA 81, 5966-5969], LTB4 was found to stimulate PI cycle activity in the macrophage (transient decrease in phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 4-phosphate, transient elevation of 1,2-diacylglycerol and elevation of phosphatidic acid) similar to the stimulation of the PI cycle by the chemotactic formyi peptides. Preincubation of macrophages with 1 μ/ml of pertussis toxin, for 1 h at 37°C, blocked LTB4 and formyl peptide stimulated O2- production and PI cycle activity. Therefore, both stimulants are proposed to act through the same coupling protein to activate phospholipase C in the early stages of macrophage activation.

AB - The influence of leukotriene B4 (LTB4) on phosphatidylinositol (PI) cycle activity was investigated in the guinea pig alveolar macrophage. In contrast to the observation reported in leukocytes [(1984) Proc. Natl. Acad. Sci. USA 81, 5966-5969], LTB4 was found to stimulate PI cycle activity in the macrophage (transient decrease in phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 4-phosphate, transient elevation of 1,2-diacylglycerol and elevation of phosphatidic acid) similar to the stimulation of the PI cycle by the chemotactic formyi peptides. Preincubation of macrophages with 1 μ/ml of pertussis toxin, for 1 h at 37°C, blocked LTB4 and formyl peptide stimulated O2- production and PI cycle activity. Therefore, both stimulants are proposed to act through the same coupling protein to activate phospholipase C in the early stages of macrophage activation.

KW - Formyl peptide

KW - Leukotriene B

KW - Macrophage

KW - Pertussis toxin

KW - Phosphatidylinositol

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Leukotriene B₄ stimulation of phosphatidylinositol turnover in macrophages and inhibition by pertussis toxin

Abstract

Keywords

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