Leukotriene B4 stimulation of phosphatidylinositol turnover in macrophages and inhibition by pertussis toxin

Research output: Contribution to journalArticlepeer-review

Abstract

The influence of leukotriene B4 (LTB4) on phosphatidylinositol (PI) cycle activity was investigated in the guinea pig alveolar macrophage. In contrast to the observation reported in leukocytes [(1984) Proc. Natl. Acad. Sci. USA 81, 5966-5969], LTB4 was found to stimulate PI cycle activity in the macrophage (transient decrease in phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 4-phosphate, transient elevation of 1,2-diacylglycerol and elevation of phosphatidic acid) similar to the stimulation of the PI cycle by the chemotactic formyi peptides. Preincubation of macrophages with 1 μ/ml of pertussis toxin, for 1 h at 37°C, blocked LTB4 and formyl peptide stimulated O2- production and PI cycle activity. Therefore, both stimulants are proposed to act through the same coupling protein to activate phospholipase C in the early stages of macrophage activation.

Original languageEnglish
Pages (from-to)15-19
Number of pages5
JournalFEBS Letters
Volume201
Issue number1
DOIs
StatePublished - May 26 1986

Keywords

  • Formyl peptide
  • Leukotriene B
  • Macrophage
  • Pertussis toxin
  • Phosphatidylinositol

Fingerprint

Dive into the research topics of 'Leukotriene B4 stimulation of phosphatidylinositol turnover in macrophages and inhibition by pertussis toxin'. Together they form a unique fingerprint.

Cite this