Lipase-mediated purification of methyl nonactate, an important natural product building block for diversity-oriented synthesis

Jennifer M. Dinges, Bradley A. Bessette, James E. Cox, Casey R. Redder, Nigel D. Priestley

Research output: Contribution to journalArticlepeer-review

Abstract

Methyl nonactate is a valuable starting material for the production of natural product-like combinatorial libraries and ketide amino acids, a series of novel conformationally constrained amino acid analogues. Fermentation of Streptomyces griseus generates high titers of macrotetrolide antibiotics from which methyl nonactate can be generated by methanolysis. Unfortunately, this approach generates mixtures of homologues that are not economically separable. We report a specific lipase-mediated hydrolysis of nonactate derivatives that discriminates between homologues and is the foundation of an economically tractable preparation of methyl nonactate in scale.

Original languageEnglish
Pages (from-to)1354-1357
Number of pages4
JournalBiotechnology Progress
Volume22
Issue number5
DOIs
StatePublished - Sep 2006

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