Abstract
Investigations of factors influencing cell division and cell shape in bacteria are commonly performed in conjunction with high-resolution fluorescence microscopy as observations made at a population level may not truly reflect what occurs at a single cell level. Live-cell timelapse microscopy allows investigators to monitor the changes in cell division or cell morphology which provide valuable insights regarding subcellular localization of proteins and timing of gene expression, as it happens, to potentially aid in answering important biological questions. Here, we describe our protocol to monitor phenotypic changes in Bacillus subtilis and Staphylococcus aureus using a high-resolution deconvolution microscope. The objective of this report is to provide a simple and clear protocol that can be adopted by other investigators interested in conducting fluorescence microscopy experiments to study different biological processes in bacteria as well as other organisms.
| Original language | English |
|---|---|
| Article number | e59905 |
| Journal | Journal of Visualized Experiments |
| Volume | 2019 |
| Issue number | 153 |
| DOIs | |
| State | Published - Nov 2019 |
Funding
We thank our lab members for their comments on this article. This work was funded by a start-up grant from the University of South Florida (PE).
| Funders | Funder number |
|---|---|
| R35GM133617 | |
| University of South Florida |
Keywords
- Bacillus subtilis
- Deconvolution microscopy
- Fluorescence microscopy
- FtsZ
- GFP
- GpsB
- Immunology and Infection
- Issue 153
- PC190723
- Protein localization
- Staphylococcus aureus
- Timelapse microscopy