Long-term quercetin dietary enrichment decreases muscle injury in mdx mice

Katrin Hollinger, R. Andrew Shanely, John C. Quindry, Joshua T. Selsby

Research output: Contribution to journalArticlepeer-review

36 Scopus citations


Background & aims: Duchenne muscular dystrophy results from a mutation in the dystrophin gene, which leads to a dystrophin-deficiency. Dystrophic muscle is marked by progressive muscle injury and loss of muscle fibers. Activation of the PGC-1α pathway has been previously shown to decrease disease-related muscle damage. Oral administration of the flavonol, quercetin, appears to be an effective and safe method to activate the PGC-1α pathway. The aim of this investigation was to determine the extent to which long term dietary quercetin enrichment would decrease muscle injury in dystrophic skeletal muscle. We hypothesized that a quercetin enriched diet would rescue dystrophic muscle from further decline and increase utrophin abundance. Methods: Beginning at three-months of age and continuing to nine-months of age mdx mice (n=10/group) were assigned to either to mdx-control receiving standard chow or to mdx-quercetin receiving a 0.2% quercetin-enriched diet. At nine-months of age mice were sacrificed and costal diaphragms collected. One hemidiaphragm was used for histological analysis and the second hemidiaphragm was used to determine gene expression via RT-qPCR. Results: The diaphragm from the mdx-quercetin group had 24% (p≤0.05) more muscle fibers/area and 34% (p≤0.05) fewer centrally nucleated fibers compared to the mdx-control group. Further, there were 44% (p≤0.05) fewer infiltrating immune cells/area, a corresponding 31% (p≤0.05) reduction in TNF gene expression, and a near 50% reduction in fibrosis. The quercetin-enriched diet increased expression of genes associated with oxidative metabolism but did not increase utrophin protein abundance. Conclusions: Long-term quercetin supplementation decreased disease-related muscle injury in dystrophic skeletal muscle; however the role of PGC-1α pathway activation as a mediator of this response is unclear.

Original languageEnglish
Pages (from-to)515-522
Number of pages8
JournalClinical Nutrition
Issue number3
StatePublished - Jun 1 2015


  • Diaphragm
  • Duchenne muscular dystrophy
  • Dystrophin
  • PGC-1α
  • Resveratrol


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