Low genotyping error rates in wild ungulate faeces sampled in winter

C. Maudet; G. Luikart; D. Dubray; A. Von Hardenberg; P. Taberlet

doi:10.1111/j.1471-8286.2004.00787.x

Low genotyping error rates in wild ungulate faeces sampled in winter

C. Maudet, G. Luikart, D. Dubray, A. Von Hardenberg, P. Taberlet

Flathead Lake Biological Station

Research output: Contribution to journal › Article › peer-review

95 Scopus citations

Abstract

We show that Alpine ibex (Capra ibex) and Corsican mouflon (Ovis musimon) faeces yield useful DNA for microsatellite analysis, however, we detected higher genotyping error rates for spring faeces than for winter faeces. We quantified the genotyping error rate by repeatedly genotyping four microsatellites. Respectively, 99 and 95% of mouflon and ibex genotyping repetitions provided a correct genotype using winter samples, whereas spring samples provided only 52 and 59% correct genotypes. Thus, before starting a noninvasive study, we recommend that researchers conduct a pilot study to quantify genotyping error rates for each season, population and species to be studied.

Original language	English
Pages (from-to)	772-775
Number of pages	4
Journal	Molecular Ecology Notes
Volume	4
Issue number	4
DOIs	https://doi.org/10.1111/j.1471-8286.2004.00787.x
State	Published - Dec 2004

Keywords

Allelic dropout
Faeces
Ibex
Microsatellites
Mouflon
Noninvasive

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10.1111/j.1471-8286.2004.00787.x

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title = "Low genotyping error rates in wild ungulate faeces sampled in winter",

abstract = "We show that Alpine ibex (Capra ibex) and Corsican mouflon (Ovis musimon) faeces yield useful DNA for microsatellite analysis, however, we detected higher genotyping error rates for spring faeces than for winter faeces. We quantified the genotyping error rate by repeatedly genotyping four microsatellites. Respectively, 99 and 95% of mouflon and ibex genotyping repetitions provided a correct genotype using winter samples, whereas spring samples provided only 52 and 59% correct genotypes. Thus, before starting a noninvasive study, we recommend that researchers conduct a pilot study to quantify genotyping error rates for each season, population and species to be studied.",

keywords = "Allelic dropout, Faeces, Ibex, Microsatellites, Mouflon, Noninvasive",

author = "C. Maudet and G. Luikart and D. Dubray and {Von Hardenberg}, A. and P. Taberlet",

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T1 - Low genotyping error rates in wild ungulate faeces sampled in winter

AU - Maudet, C.

AU - Luikart, G.

AU - Dubray, D.

AU - Von Hardenberg, A.

AU - Taberlet, P.

PY - 2004/12

Y1 - 2004/12

N2 - We show that Alpine ibex (Capra ibex) and Corsican mouflon (Ovis musimon) faeces yield useful DNA for microsatellite analysis, however, we detected higher genotyping error rates for spring faeces than for winter faeces. We quantified the genotyping error rate by repeatedly genotyping four microsatellites. Respectively, 99 and 95% of mouflon and ibex genotyping repetitions provided a correct genotype using winter samples, whereas spring samples provided only 52 and 59% correct genotypes. Thus, before starting a noninvasive study, we recommend that researchers conduct a pilot study to quantify genotyping error rates for each season, population and species to be studied.

AB - We show that Alpine ibex (Capra ibex) and Corsican mouflon (Ovis musimon) faeces yield useful DNA for microsatellite analysis, however, we detected higher genotyping error rates for spring faeces than for winter faeces. We quantified the genotyping error rate by repeatedly genotyping four microsatellites. Respectively, 99 and 95% of mouflon and ibex genotyping repetitions provided a correct genotype using winter samples, whereas spring samples provided only 52 and 59% correct genotypes. Thus, before starting a noninvasive study, we recommend that researchers conduct a pilot study to quantify genotyping error rates for each season, population and species to be studied.

KW - Allelic dropout

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KW - Ibex

KW - Microsatellites

KW - Mouflon

KW - Noninvasive

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JO - Molecular Ecology Notes

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Low genotyping error rates in wild ungulate faeces sampled in winter

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Keywords

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