TY - JOUR
T1 - MEDUSA
T2 - A cloud-based tool for the analysis of X-ray diffuse scattering to obtain the bending modulus from oriented membrane stacks
AU - Himbert, Sebastian
AU - Gaboo, Dorian
AU - Brookes, Emre
AU - Nagle, John F.
AU - Rheinstädter, Maikel C.
N1 - Copyright: © 2024 Himbert et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2024/1/8
Y1 - 2024/1/8
N2 - An important mechanical property of cells is their membrane bending modulus, κ. Here, we introduce MEDUSA (MEmbrane DiffUse Scattering Analysis), a cloud-based analysis tool to determine the bending modulus, κ, from the analysis of X-ray diffuse scattering. MEDUSA uses GPU (graphics processing unit) accelerated hardware and a parallelized algorithm to run the calculations efficiently in a few seconds. MEDUSA’s graphical user interface allows the user to upload 2-dimensional data collected from different sources, perform background subtraction and distortion corrections, select regions of interest, run the fitting procedure and output the fitted parameters, the membranes’ bending modulus κ, and compressional modulus B.
AB - An important mechanical property of cells is their membrane bending modulus, κ. Here, we introduce MEDUSA (MEmbrane DiffUse Scattering Analysis), a cloud-based analysis tool to determine the bending modulus, κ, from the analysis of X-ray diffuse scattering. MEDUSA uses GPU (graphics processing unit) accelerated hardware and a parallelized algorithm to run the calculations efficiently in a few seconds. MEDUSA’s graphical user interface allows the user to upload 2-dimensional data collected from different sources, perform background subtraction and distortion corrections, select regions of interest, run the fitting procedure and output the fitted parameters, the membranes’ bending modulus κ, and compressional modulus B.
KW - Algorithms
KW - Cloud Computing
KW - Radiography
KW - X-Rays
UR - http://www.scopus.com/inward/record.url?scp=85182956558&partnerID=8YFLogxK
U2 - 10.1371/journal.pcbi.1011749
DO - 10.1371/journal.pcbi.1011749
M3 - Article
C2 - 38190400
AN - SCOPUS:85182956558
SN - 1553-734X
VL - 20
SP - e1011749
JO - PLoS Computational Biology
JF - PLoS Computational Biology
IS - 1
M1 - e1011749
ER -