Abstract
Analytical ultracentrifugation experiments play an integral role in the solution-phase characterization of biological macromolecules and their interactions. This unit discusses the design of sedimentation velocity and sedimentation equilibrium experiments performed with a Beckman Proteomelab XL-A or XL-I analytical ultracentrifuge and with a Beckman Optima AUC. Instrument settings and experimental design considerations are explained, and strategies for the analysis of experimental data with the UltraScan data analysis software package are presented. Special attention is paid to the strengths and weaknesses of the available detectors, and guidance is provided on how to extract maximum information from analytical ultracentrifugation experiments.
| Original language | English |
|---|---|
| Article number | e974 |
| Journal | Current Protocols |
| Volume | 4 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 2024 |
Funding
This work was performed at the Canadian Center for Hydrodynamics at the University of Lethbridge and supported by grants to the author, including from the Canada 150 Research Chairs program (C150‐2017‐00015), the US National Institutes of Health (GM‐120600), and the Canadian Natural Science and Engineering Research Council (DG‐RGPIN‐2019‐05637). The Canadian Center for Hydrodynamics is funded by the Canada Foundation for Innovation (CFI‐37589). UltraScan supercomputer calculations were supported through NSF/ACCESS (MCB070039N) and the University of Texas (TG457201).
| Funders | Funder number |
|---|---|
| MCB070039N | |
| GM‐120600 | |
| University of Texas at Austin | TG457201 |
| DG‐RGPIN‐2019‐05637 | |
| Canada Foundation for Innovation | CFI‐37589 |
Keywords
- 2-dimensional spectrum analysis
- AAV capsid quantification
- absorbance optics
- analytical buoyant-density equilibrium
- analytical ultracentrifugation
- composition analysis
- custom grid method
- experimental design
- intensity measurements
- interaction studies
- Monte Carlo method
- multi-wavelength analytical ultracentrifugation
- parametrically constrained spectrum analysis
- sedimentation velocity
- solution studies
- stoichiometry determination
- UltraScan
- van Holde–Weischet analysis
- Ultracentrifugation/methods
- Research Design