Micronucleus-centromere assay and DNA repair gene polymorphism in lymphocytes of industrial radiographers

Yoon Hee Cho; Yang Jee Kim; You Sun An; Hae Dong Woo; Soo Yong Choi; Chang Mo Kang; Hai Won Chung

doi:10.1016/j.mrgentox.2009.08.007

Micronucleus-centromere assay and DNA repair gene polymorphism in lymphocytes of industrial radiographers

Yoon Hee Cho, Yang Jee Kim, You Sun An, Hae Dong Woo, Soo Yong Choi, Chang Mo Kang, Hai Won Chung

Center for Environmental Health Sciences

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

The micronucleus-centromere assay using a pan-centromeric probe was used to assess chromosomal damage in lymphocytes of 47 industrial radiographers occupationally exposed to low dose ionizing radiation and 47 controls. The influence of genotype of DNA repair genes (XRCC1³⁹⁹, XRCC3²⁴¹ and XPD⁷⁵¹) on micronuclei (MN) frequency was also investigated. Centromere negative micronuclei (MNC-) frequency was significantly higher in radiographers than in controls, whereas similar centromere positive micronuclei (MNC+) frequency was observed in both groups. Poisson regression analyses revealed that the MNC- frequency was significantly associated with radiation occupational exposure and with cumulative-radiation doses in radiographers, after adjusting for confounding variables such as age, smoking, alcohol intake and genotypes. Compared to homozygous wild-type subjects, MNC- frequency in radiographers with variant XRCC3 genotype was significantly higher using univariate analysis. There were no differences in MNC- or MNC+ frequencies by genotype in controls. In conclusion, scoring of MNC- is a useful cytogenetic biomonitoring method for radiographers. Polymorphisms in XRCC3 might contribute to the increased genetic damage in individuals occupationally exposed to chronic ionizing radiation.

Original language	English
Pages (from-to)	17-24
Number of pages	8
Journal	Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume	680
Issue number	1-2
DOIs	https://doi.org/10.1016/j.mrgentox.2009.08.007
State	Published - Nov 2009

Keywords

Centromere negative micronuclei
DNA repair genes
Micronucleus-centromere assay
Polymorphism
Radiographers

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10.1016/j.mrgentox.2009.08.007

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title = "Micronucleus-centromere assay and DNA repair gene polymorphism in lymphocytes of industrial radiographers",

abstract = "The micronucleus-centromere assay using a pan-centromeric probe was used to assess chromosomal damage in lymphocytes of 47 industrial radiographers occupationally exposed to low dose ionizing radiation and 47 controls. The influence of genotype of DNA repair genes (XRCC1399, XRCC3241 and XPD751) on micronuclei (MN) frequency was also investigated. Centromere negative micronuclei (MNC-) frequency was significantly higher in radiographers than in controls, whereas similar centromere positive micronuclei (MNC+) frequency was observed in both groups. Poisson regression analyses revealed that the MNC- frequency was significantly associated with radiation occupational exposure and with cumulative-radiation doses in radiographers, after adjusting for confounding variables such as age, smoking, alcohol intake and genotypes. Compared to homozygous wild-type subjects, MNC- frequency in radiographers with variant XRCC3 genotype was significantly higher using univariate analysis. There were no differences in MNC- or MNC+ frequencies by genotype in controls. In conclusion, scoring of MNC- is a useful cytogenetic biomonitoring method for radiographers. Polymorphisms in XRCC3 might contribute to the increased genetic damage in individuals occupationally exposed to chronic ionizing radiation.",

keywords = "Centromere negative micronuclei, DNA repair genes, Micronucleus-centromere assay, Polymorphism, Radiographers",

author = "Cho, {Yoon Hee} and Kim, {Yang Jee} and An, {You Sun} and Woo, {Hae Dong} and Choi, {Soo Yong} and Kang, {Chang Mo} and Chung, {Hai Won}",

note = "Funding Information: This work was supported by the Nuclear R&D Program of the Ministry of Sciences and Technology of Korea (M20504050002-05A0705-00211) and the Brain Korea 21 Project in 2008. ",

year = "2009",

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doi = "10.1016/j.mrgentox.2009.08.007",

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AU - Cho, Yoon Hee

AU - Kim, Yang Jee

AU - An, You Sun

AU - Woo, Hae Dong

AU - Choi, Soo Yong

AU - Kang, Chang Mo

AU - Chung, Hai Won

N1 - Funding Information: This work was supported by the Nuclear R&D Program of the Ministry of Sciences and Technology of Korea (M20504050002-05A0705-00211) and the Brain Korea 21 Project in 2008.

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Y1 - 2009/11

N2 - The micronucleus-centromere assay using a pan-centromeric probe was used to assess chromosomal damage in lymphocytes of 47 industrial radiographers occupationally exposed to low dose ionizing radiation and 47 controls. The influence of genotype of DNA repair genes (XRCC1399, XRCC3241 and XPD751) on micronuclei (MN) frequency was also investigated. Centromere negative micronuclei (MNC-) frequency was significantly higher in radiographers than in controls, whereas similar centromere positive micronuclei (MNC+) frequency was observed in both groups. Poisson regression analyses revealed that the MNC- frequency was significantly associated with radiation occupational exposure and with cumulative-radiation doses in radiographers, after adjusting for confounding variables such as age, smoking, alcohol intake and genotypes. Compared to homozygous wild-type subjects, MNC- frequency in radiographers with variant XRCC3 genotype was significantly higher using univariate analysis. There were no differences in MNC- or MNC+ frequencies by genotype in controls. In conclusion, scoring of MNC- is a useful cytogenetic biomonitoring method for radiographers. Polymorphisms in XRCC3 might contribute to the increased genetic damage in individuals occupationally exposed to chronic ionizing radiation.

AB - The micronucleus-centromere assay using a pan-centromeric probe was used to assess chromosomal damage in lymphocytes of 47 industrial radiographers occupationally exposed to low dose ionizing radiation and 47 controls. The influence of genotype of DNA repair genes (XRCC1399, XRCC3241 and XPD751) on micronuclei (MN) frequency was also investigated. Centromere negative micronuclei (MNC-) frequency was significantly higher in radiographers than in controls, whereas similar centromere positive micronuclei (MNC+) frequency was observed in both groups. Poisson regression analyses revealed that the MNC- frequency was significantly associated with radiation occupational exposure and with cumulative-radiation doses in radiographers, after adjusting for confounding variables such as age, smoking, alcohol intake and genotypes. Compared to homozygous wild-type subjects, MNC- frequency in radiographers with variant XRCC3 genotype was significantly higher using univariate analysis. There were no differences in MNC- or MNC+ frequencies by genotype in controls. In conclusion, scoring of MNC- is a useful cytogenetic biomonitoring method for radiographers. Polymorphisms in XRCC3 might contribute to the increased genetic damage in individuals occupationally exposed to chronic ionizing radiation.

KW - Centromere negative micronuclei

KW - DNA repair genes

KW - Micronucleus-centromere assay

KW - Polymorphism

KW - Radiographers

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ER -

Micronucleus-centromere assay and DNA repair gene polymorphism in lymphocytes of industrial radiographers

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