Modified low density lipoproteins binding requires a lysine cluster region in the murine macrophage scavenger receptor class A type II

Francisco J. Leyva, Mark A. Pershouse, Andrij Holian

Research output: Contribution to journalArticlepeer-review

Abstract

Atherosclerosis is a consequence of lipid deposition and foam cell formation in the arterial wall. Macrophage scavenger receptor A II is involved in the uptake of modified low density lipoproteins. It contains an extracellular conserved lysine cluster which has been proposed to form a positively charged groove that interacts with acetylated low density lipoproteins (AcLDL). This study evaluated the role of the murine SRA-II and a lysine mutated SRA-II on AcLDL uptake. Fluorescence labeled AcLDL uptake was quantified using a Laser Scan Cytometer. A significant increase in fluorescence uptake was found in the cells transfected with SRA-II versus those with empty vector. Cells expressing the lysine mutated SRA-II also demonstrated a significant decrease in their uptake of AcLDL. This data supports the concept that the conserved lysine cluster in murine SRA-II is the binding region for AcLDL or contributes to the trimeric structure of SRA-II necessary for AcLDL binding.

Original languageEnglish
Pages (from-to)2847-2852
Number of pages6
JournalMolecular Biology Reports
Volume37
Issue number6
DOIs
StatePublished - Jul 2010

Keywords

  • Atherosclerosis
  • Foam cells
  • Low density lipoproteins
  • Macrophage
  • Scavenger receptors

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