Modified low density lipoproteins binding requires a lysine cluster region in the murine macrophage scavenger receptor class A type II

Francisco J. Leyva; Mark A. Pershouse; Andrij Holian

doi:10.1007/s11033-009-9837-3

Modified low density lipoproteins binding requires a lysine cluster region in the murine macrophage scavenger receptor class A type II

Francisco J. Leyva
, Mark A. Pershouse
, Andrij Holian

National Institutes of Health

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Atherosclerosis is a consequence of lipid deposition and foam cell formation in the arterial wall. Macrophage scavenger receptor A II is involved in the uptake of modified low density lipoproteins. It contains an extracellular conserved lysine cluster which has been proposed to form a positively charged groove that interacts with acetylated low density lipoproteins (AcLDL). This study evaluated the role of the murine SRA-II and a lysine mutated SRA-II on AcLDL uptake. Fluorescence labeled AcLDL uptake was quantified using a Laser Scan Cytometer. A significant increase in fluorescence uptake was found in the cells transfected with SRA-II versus those with empty vector. Cells expressing the lysine mutated SRA-II also demonstrated a significant decrease in their uptake of AcLDL. This data supports the concept that the conserved lysine cluster in murine SRA-II is the binding region for AcLDL or contributes to the trimeric structure of SRA-II necessary for AcLDL binding.

Original language	English
Pages (from-to)	2847-2852
Number of pages	6
Journal	Molecular Biology Reports
Volume	37
Issue number	6
DOIs	https://doi.org/10.1007/s11033-009-9837-3
State	Published - Jul 2010

Funding

Acknowledgments This publication was made possible by Grant Number P20-RR-017670 from the National Center for Research Resources (NCRR), a component of the National Institutes of Health (NIH). Its contents are solely the responsibility of the authors and do not necessarily represent the official views of NCRR or NIH. Additional funding for this research was provided by Grant Number ES-04804 from the NIEHS.

Funder number
R01ES004804
P20RR017670

Keywords

Atherosclerosis
Foam cells
Low density lipoproteins
Macrophage
Scavenger receptors

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10.1007/s11033-009-9837-3

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title = "Modified low density lipoproteins binding requires a lysine cluster region in the murine macrophage scavenger receptor class A type II",

abstract = "Atherosclerosis is a consequence of lipid deposition and foam cell formation in the arterial wall. Macrophage scavenger receptor A II is involved in the uptake of modified low density lipoproteins. It contains an extracellular conserved lysine cluster which has been proposed to form a positively charged groove that interacts with acetylated low density lipoproteins (AcLDL). This study evaluated the role of the murine SRA-II and a lysine mutated SRA-II on AcLDL uptake. Fluorescence labeled AcLDL uptake was quantified using a Laser Scan Cytometer. A significant increase in fluorescence uptake was found in the cells transfected with SRA-II versus those with empty vector. Cells expressing the lysine mutated SRA-II also demonstrated a significant decrease in their uptake of AcLDL. This data supports the concept that the conserved lysine cluster in murine SRA-II is the binding region for AcLDL or contributes to the trimeric structure of SRA-II necessary for AcLDL binding.",

keywords = "Atherosclerosis, Foam cells, Low density lipoproteins, Macrophage, Scavenger receptors",

author = "Leyva, \{Francisco J.\} and Pershouse, \{Mark A.\} and Andrij Holian",

note = "Funding Information: Acknowledgments This publication was made possible by Grant Number P20-RR-017670 from the National Center for Research Resources (NCRR), a component of the National Institutes of Health (NIH). Its contents are solely the responsibility of the authors and do not necessarily represent the official views of NCRR or NIH. Additional funding for this research was provided by Grant Number ES-04804 from the NIEHS.",

year = "2010",

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doi = "10.1007/s11033-009-9837-3",

language = "English",

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AU - Leyva, Francisco J.

AU - Pershouse, Mark A.

AU - Holian, Andrij

N1 - Funding Information: Acknowledgments This publication was made possible by Grant Number P20-RR-017670 from the National Center for Research Resources (NCRR), a component of the National Institutes of Health (NIH). Its contents are solely the responsibility of the authors and do not necessarily represent the official views of NCRR or NIH. Additional funding for this research was provided by Grant Number ES-04804 from the NIEHS.

PY - 2010/7

Y1 - 2010/7

N2 - Atherosclerosis is a consequence of lipid deposition and foam cell formation in the arterial wall. Macrophage scavenger receptor A II is involved in the uptake of modified low density lipoproteins. It contains an extracellular conserved lysine cluster which has been proposed to form a positively charged groove that interacts with acetylated low density lipoproteins (AcLDL). This study evaluated the role of the murine SRA-II and a lysine mutated SRA-II on AcLDL uptake. Fluorescence labeled AcLDL uptake was quantified using a Laser Scan Cytometer. A significant increase in fluorescence uptake was found in the cells transfected with SRA-II versus those with empty vector. Cells expressing the lysine mutated SRA-II also demonstrated a significant decrease in their uptake of AcLDL. This data supports the concept that the conserved lysine cluster in murine SRA-II is the binding region for AcLDL or contributes to the trimeric structure of SRA-II necessary for AcLDL binding.

AB - Atherosclerosis is a consequence of lipid deposition and foam cell formation in the arterial wall. Macrophage scavenger receptor A II is involved in the uptake of modified low density lipoproteins. It contains an extracellular conserved lysine cluster which has been proposed to form a positively charged groove that interacts with acetylated low density lipoproteins (AcLDL). This study evaluated the role of the murine SRA-II and a lysine mutated SRA-II on AcLDL uptake. Fluorescence labeled AcLDL uptake was quantified using a Laser Scan Cytometer. A significant increase in fluorescence uptake was found in the cells transfected with SRA-II versus those with empty vector. Cells expressing the lysine mutated SRA-II also demonstrated a significant decrease in their uptake of AcLDL. This data supports the concept that the conserved lysine cluster in murine SRA-II is the binding region for AcLDL or contributes to the trimeric structure of SRA-II necessary for AcLDL binding.

KW - Atherosclerosis

KW - Foam cells

KW - Low density lipoproteins

KW - Macrophage

KW - Scavenger receptors

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JO - Molecular Biology Reports

JF - Molecular Biology Reports

IS - 6

ER -

Modified low density lipoproteins binding requires a lysine cluster region in the murine macrophage scavenger receptor class A type II

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