Molecular evidence for a new bacteriophage of Borrelia burgdorferi

Christian H. Eggers, D. Scott Samuels

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103 Scopus citations

Abstract

We have recovered a DNase-protected, chloroform-resistant molecule of DNA from the cell-free supernatant of a Borrelia burgdorferi culture. The DNA is a 32-kb double-stranded linear molecule that is derived from the 32-kb circular plasmids (cp32s) of the B. burgdorferi genome. Electron microscopy of samples from which the 32-kb DNA molecule was purified revealed bacteriophage particles. The bacteriophage has a polyhedral head with a diameter of 55 nm and appears to have a simple 100-nm-long tail. The phage is produced constitutively at low levels from growing cultures of some B. burgdorferi strains and is inducible to higher levels with 10 μg of 1-methyl-3-nitroso-nitroguanidine (MNNG) ml-1. In addition, the prophage can be induced with MNNG from some Borrelia isolates that do not naturally produce phage. We have isolated and partially characterized the phage associated with B. burgdorferi CA-11.2A. To our knowledge, this is the first molecular characterization of a bacteriophage of B. burgdorferi.

Original languageEnglish
Pages (from-to)7308-7313
Number of pages6
JournalJournal of Bacteriology
Volume181
Issue number23
DOIs
StatePublished - Dec 1999

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