MRI-guided dissection of the nonhuman primate brain: A case study

James Bernard Daunais, Robert Arthur Kraft, April Teresa Davenport, Elizabeth J. Burnett, Vicki Moser Maxey, Kendall Thomas Szeliga, Andrew Ryan Rau, Graham Stallard Flory, Scott Edwards Hemby, Christopher David Kroenke, Kathleen Alice Grant, David Paul Friedman

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


Numerous biochemical as well as electrophysiological techniques require tissue that must be retrieved very quickly following death in order to preserve the physiological integrity of the neuronal environment. Therefore, the ability to accurately predict the precise locations of brain regions of interest (ROI) and to retrieve those areas as quickly as possible following the brain harvest is critical for subsequent analyses. One way to achieve this objective is the utilization of high-resolution MRI to guide the subsequent dissections. In the present study, individual MRI images of the brains of rhesus and cynomolgus macaques that had chronically self-administered ethanol were employed in order to determine which blocks of dissected tissue contained specific ROIs. MRI-guided brain dissection of discrete brain regions was completely accurate in 100% of the cases. In comparison, approximately 60-70% accuracy was achieved in dissections that relied on external landmarks alone without the aid of MRI. These results clearly demonstrate that the accuracy of targeting specific brain areas can be improved with high-resolution MR imaging.

Original languageEnglish
Pages (from-to)199-204
Number of pages6
Issue number3
StatePublished - Mar 2010


  • Brain
  • Dissection
  • Ethanol
  • Magnetic resonance imaging
  • Primate
  • Region of interest


Dive into the research topics of 'MRI-guided dissection of the nonhuman primate brain: A case study'. Together they form a unique fingerprint.

Cite this