Multiple levels for regulation of TrkA in PC12 cells by nerve growth factor

Jie Zhou, Janice S. Valletta, Mark L. Grimes, William C. Mobley

Research output: Contribution to journalArticlepeer-review

Abstract

TrkA is a receptor tyrosine kinase for nerve growth factor (NGF). Recent studies indicate that NGF regulates not only activation of trkA kinase but also expression of the trkA gene. To further define NGF actions on trkA, we examined binding and signaling through trkA after both short and long intervals of NGF treatment. Induction of tyrosine phosphorylation on gp140(trkA) was rapidly followed by down-regulation of cell surface and total cellular gp140(trkA). At later intervals, increased expression of trkA was evident in increased mRNA and protein levels. At 7 days, there was increased binding to gp140(trkA) and increased signaling through this receptor. NGF appears to regulate trkA at several levels. In neurons persistently exposed to NGF, maintenance of NGF signaling may require increased trkA gene expression.

Original languageEnglish
Pages (from-to)1146-1156
Number of pages11
JournalJournal of Neurochemistry
Volume65
Issue number3
StatePublished - Sep 1995

Keywords

  • Extracellular signal-regulated kinase-1
  • Phospholipase C- γ1
  • Phosphorylation
  • Receptor
  • Signaling
  • Tyrosine kinase

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