TY - JOUR
T1 - Muscle cytokine mRNA changes after 2.5 h of cycling
T2 - Influence of carbohydrate
AU - Nieman, David C.
AU - Davis, J. Mark
AU - Henson, Dru A.
AU - Gross, Sarah J.
AU - Dumke, Charles L.
AU - Utter, Alan C.
AU - Vinci, Debra M.
AU - Carson, James A.
AU - Brown, Adrienne
AU - McAnulty, Steve R.
AU - McAnulty, Lisa S.
AU - Triplett, N. Travis
PY - 2005/8
Y1 - 2005/8
N2 - Purpose: To study the effect of carbohydrate compared to placebo ingestion on plasma cytokines and muscle cytokine mRNA following 2.5 h of intensive cycling in 15 trained cyclists. Methods: Fifteen trained cyclists cycled for 2.5 h at 60% Wmax on two occasions while receiving 4 mL̇kg -1̇15 min-1 carbohydrate (6%) (CHO) or placebo (PLA) beverages in a randomized, counterbalanced design. Blood and vastus lateralis muscle biopsy samples were collected before and after exercise and 12 h postexercise and compared to samples taken from five cyclists who rested in the lab during the exercise sessions. Blood cell counts were determined, and plasma was analyzed for interleukin (IL)-6, IL-10, IL-1 receptor antagonist (ra), IL-8, cortisol, epinephrine, glucose, and insulin. Muscle was analyzed for glycogen content and relative gene expression of four cytokines, IL-6, IL-8, tumor necrosis factor (TNF) α, and IL-1β, using real-time quantitative reverse transcriptase polymerase chain reaction. Results: Plasma glucose and insulin were higher, and epinephrine, cortisol, IL-6, IL-10, and IL-1ra, but not IL-8, were significantly lower postexercise in CHO versus PLA. Muscle glycogen content decreased 68% immediately postexercise and the pattern of change did not differ between CHO and PLA. Muscle IL-6, IL-8, TNF-α, but not IL-1β mRNA increased immediately postexercise compared to controls, with no differences between CHO and PLA. Conclusion: CHO compared to PLA beverage ingestion attenuated the increase in plasma cortisol, epinephrine, IL-6, IL-10, and IL-1ra, but not muscle IL-6, IL-8, and TNF-α mRNA in athletes cycling 2.5 h at 60% Wmax.
AB - Purpose: To study the effect of carbohydrate compared to placebo ingestion on plasma cytokines and muscle cytokine mRNA following 2.5 h of intensive cycling in 15 trained cyclists. Methods: Fifteen trained cyclists cycled for 2.5 h at 60% Wmax on two occasions while receiving 4 mL̇kg -1̇15 min-1 carbohydrate (6%) (CHO) or placebo (PLA) beverages in a randomized, counterbalanced design. Blood and vastus lateralis muscle biopsy samples were collected before and after exercise and 12 h postexercise and compared to samples taken from five cyclists who rested in the lab during the exercise sessions. Blood cell counts were determined, and plasma was analyzed for interleukin (IL)-6, IL-10, IL-1 receptor antagonist (ra), IL-8, cortisol, epinephrine, glucose, and insulin. Muscle was analyzed for glycogen content and relative gene expression of four cytokines, IL-6, IL-8, tumor necrosis factor (TNF) α, and IL-1β, using real-time quantitative reverse transcriptase polymerase chain reaction. Results: Plasma glucose and insulin were higher, and epinephrine, cortisol, IL-6, IL-10, and IL-1ra, but not IL-8, were significantly lower postexercise in CHO versus PLA. Muscle glycogen content decreased 68% immediately postexercise and the pattern of change did not differ between CHO and PLA. Muscle IL-6, IL-8, TNF-α, but not IL-1β mRNA increased immediately postexercise compared to controls, with no differences between CHO and PLA. Conclusion: CHO compared to PLA beverage ingestion attenuated the increase in plasma cortisol, epinephrine, IL-6, IL-10, and IL-1ra, but not muscle IL-6, IL-8, and TNF-α mRNA in athletes cycling 2.5 h at 60% Wmax.
KW - Cortisol
KW - Gene expression
KW - Muscle glycogen
KW - Real-time quantitative RT-PCR
UR - http://www.scopus.com/inward/record.url?scp=23944505949&partnerID=8YFLogxK
U2 - 10.1249/01.mss.0000175054.99588.b1
DO - 10.1249/01.mss.0000175054.99588.b1
M3 - Article
C2 - 16118573
AN - SCOPUS:23944505949
SN - 0195-9131
VL - 37
SP - 1283
EP - 1290
JO - Medicine and Science in Sports and Exercise
JF - Medicine and Science in Sports and Exercise
IS - 8
ER -