Abstract
Nonactin is the parent compound of a group of highly atypical polyketide metabolites produced by Streptomyces griseus subsp. griseus ETH A7796. In this paper we describe the isolation, sequencing, and analysis of 15 559 bp of chromosomal DNA, containing the potential nonactin biosynthesis gene cluster, from S. griseus subsp. griseus ETH A7796. Fourteen open reading frames were observed in the DNA sequence. Significantly, type II polyketide synthase (PKS) homologues were discovered in an apparent operon structure, which also contained the nonactate synthase gene (nonS), clustered with the tetranactin resistance gene. The deduced products of two of the genes (nonK and nonJ) are quite unusual ketoacyl synthase (KAS) α and KASβ homologues. We speculate that nonactic acid, the polyketide precursor of nonactin, is synthesized by a type II PKS system. (C) Federation of European Microbiological Societies.
| Original language | English |
|---|---|
| Pages (from-to) | 171-175 |
| Number of pages | 5 |
| Journal | FEMS Microbiology Letters |
| Volume | 183 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 1 2000 |
Funding
We especially appreciate the support and technical assistance of Don Ordaz and the OSU Fermentation Facility. This work was supported by the College of Pharmacy, The Ohio State University, and by Grant CA77347 from the National Cancer Institute. Dr. Savita Shanker of the DNA Sequencing Core Laboratory at the University of Florida is thanked for providing sequencing facilities.
| Funders | Funder number |
|---|---|
| R29CA077347 | |
| Ohio State University |
Keywords
- Biosynthesis
- Ketoacyl synthase
- Macrotetrolide
- Nonactin
- Polyketide