TY - JOUR
T1 - Particulate matter immunomodulatory effects on autoantibody development in New Zealand mixed mice
AU - Hassani, Mary
AU - Brown, Jared M.
AU - Morandi, Maria T.
AU - Holian, Andrij
N1 - Funding Information:
Received 25 February, 2004; Accepted 24 June, 2004. Address correspondence to Andrij Holian, SB 154A, Center for Environmental Health Sciences, University of Montana, Missoula, Montana 59812, USA; e-mail: [email protected] This study was supported by U.S. National Institute of Environmental Health Sciences grants ES-11120, RR-017670, and AFPE predoctoral fellowship for JMB.
PY - 2004
Y1 - 2004
N2 - Particulate matter exposures have been linked to increased mortality and morbidity that may be associated with immune dysfunction. Therefore, Lupus-prone New Zealand mixed mice (NZM) were intranasally instilled with either 30 μ? l saline or 30 μ? l saline suspensions of 500 μ? g acid-washed PM 1648, PM 1648 or PM2.5 collected in Houston, TX, once a week for 4 weeks. Lung injury, mortality, and various immune dysfuntions were assessed. Accelerated mortality was observed in the PM 1648 and PM2.5 instilled mice compared to the acid-washed PM 1648 and saline-instilled mice. PM 1648 and PM2.5 significantly suppressed the natural development of anti-nuclear antibodies in NZM mice at 16 weeks. IgG serum levels were significantly increased in the acid-washed PM 1648 instilled mice at 8 weeks following PM instillation compared to the saline-instilled group. In contrast, IgG serum levels were significantly decreased in the PM 1648 and PM2.5 instilled mice at 8 weeks post-PM instillation as compared to the acid-washed PM 1648 instilled group. There were increases in the amount of immune cell infiltration, fibrosis and collagen deposition within the lungs of PM 1648 and PM2.5 groups in comparison within the saline- and acid-washed PM 1648 instilled mice. These results demonstrate that PM has an immunosuppressive effect on the development of anti-nuclear autoantibodies and modulates the IgG responses in this model of autoimmune disease.
AB - Particulate matter exposures have been linked to increased mortality and morbidity that may be associated with immune dysfunction. Therefore, Lupus-prone New Zealand mixed mice (NZM) were intranasally instilled with either 30 μ? l saline or 30 μ? l saline suspensions of 500 μ? g acid-washed PM 1648, PM 1648 or PM2.5 collected in Houston, TX, once a week for 4 weeks. Lung injury, mortality, and various immune dysfuntions were assessed. Accelerated mortality was observed in the PM 1648 and PM2.5 instilled mice compared to the acid-washed PM 1648 and saline-instilled mice. PM 1648 and PM2.5 significantly suppressed the natural development of anti-nuclear antibodies in NZM mice at 16 weeks. IgG serum levels were significantly increased in the acid-washed PM 1648 instilled mice at 8 weeks following PM instillation compared to the saline-instilled group. In contrast, IgG serum levels were significantly decreased in the PM 1648 and PM2.5 instilled mice at 8 weeks post-PM instillation as compared to the acid-washed PM 1648 instilled group. There were increases in the amount of immune cell infiltration, fibrosis and collagen deposition within the lungs of PM 1648 and PM2.5 groups in comparison within the saline- and acid-washed PM 1648 instilled mice. These results demonstrate that PM has an immunosuppressive effect on the development of anti-nuclear autoantibodies and modulates the IgG responses in this model of autoimmune disease.
UR - http://www.scopus.com/inward/record.url?scp=80052277589&partnerID=8YFLogxK
U2 - 10.1080/15476910490505644
DO - 10.1080/15476910490505644
M3 - Article
AN - SCOPUS:80052277589
SN - 1547-691X
VL - 1
SP - 95
EP - 102
JO - Journal of Immunotoxicology
JF - Journal of Immunotoxicology
IS - 2
ER -