Peptides from cytomegalovirus UL130 and UL131 proteins induce high titer antibodies that block viral entry into mucosal epithelial cells

Frances M. Saccoccio; Anne L. Sauer; Xiaohong Cui; Amy E. Armstrong; EL Sayed E. Habib; David C. Johnson; Brent J. Ryckman; Aloysius J. Klingelhutz; Stuart P. Adler; Michael A. McVoy

doi:10.1016/j.vaccine.2011.01.079

Peptides from cytomegalovirus UL130 and UL131 proteins induce high titer antibodies that block viral entry into mucosal epithelial cells

Frances M. Saccoccio
, Anne L. Sauer
, Xiaohong Cui
, Amy E. Armstrong
, EL Sayed E. Habib
, David C. Johnson
, Brent J. Ryckman
, Aloysius J. Klingelhutz
, Stuart P. Adler
, Michael A. McVoy

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

Cytomegalovirus infections are an important cause of disease for which no licensed vaccine exists. Recent studies have focused on the gH/gL/UL128-131 complex as antibodies to gH/gL/UL128-131 neutralize viral entry into epithelial cells. Prior studies have used cells from the retinal pigment epithelium, while to prevent transmission, vaccine-induced antibodies may need to block viral infection of epithelial cells of the oral or genital mucosa. We found that gH/gL/UL128-131 is necessary for efficient viral entry into epithelial cells derived from oral and genital mucosa, that short peptides from UL130 and UL131 elicit high titer neutralizing antibodies in rabbits, and that such antibodies neutralize viral entry into epithelial cells derived from these relevant tissues. These results suggest that single subunits or peptides may be sufficient to elicit potent epithelial entry neutralizing responses and that secretory antibodies to such neutralizing epitopes have the potential to provide sterilizing immunity by blocking initial mucosal infection.

Original language	English
Pages (from-to)	2705-2711
Number of pages	7
Journal	Vaccine
Volume	29
Issue number	15
DOIs	https://doi.org/10.1016/j.vaccine.2011.01.079
State	Published - Mar 24 2011

Funding

We thank Ronzo Lee for technical assistance, Dai Wang and Thomas Shenk for providing BAD r UL131-Y4 BAC, David Gewirtz for use of the LX70 Inverted UV microscope, and Huiping Zhou for use of the Victor 3 V 1420 Multilable Counter. This work was supported in part by NIH/NIAID grant R21AI073615 (to MM), NIH/NIAID fellowship 1F31A073209 (to AS), and a Summer Research Fellowship Award from the Virginia Commonwealth University School of Medicine (to AA).

Funder number
1F31A073209
R21AI073615

Keywords

Cytomegalovirus
Mucosal infection
Neutralizing antibody
Vaccine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.vaccine.2011.01.079

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Saccoccio, F. M., Sauer, A. L., Cui, X., Armstrong, A. E., Habib, EL. S. E., Johnson, D. C., Ryckman, B. J., Klingelhutz, A. J., Adler, S. P., & McVoy, M. A. (2011). Peptides from cytomegalovirus UL130 and UL131 proteins induce high titer antibodies that block viral entry into mucosal epithelial cells. Vaccine, 29(15), 2705-2711. https://doi.org/10.1016/j.vaccine.2011.01.079

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title = "Peptides from cytomegalovirus UL130 and UL131 proteins induce high titer antibodies that block viral entry into mucosal epithelial cells",

abstract = "Cytomegalovirus infections are an important cause of disease for which no licensed vaccine exists. Recent studies have focused on the gH/gL/UL128-131 complex as antibodies to gH/gL/UL128-131 neutralize viral entry into epithelial cells. Prior studies have used cells from the retinal pigment epithelium, while to prevent transmission, vaccine-induced antibodies may need to block viral infection of epithelial cells of the oral or genital mucosa. We found that gH/gL/UL128-131 is necessary for efficient viral entry into epithelial cells derived from oral and genital mucosa, that short peptides from UL130 and UL131 elicit high titer neutralizing antibodies in rabbits, and that such antibodies neutralize viral entry into epithelial cells derived from these relevant tissues. These results suggest that single subunits or peptides may be sufficient to elicit potent epithelial entry neutralizing responses and that secretory antibodies to such neutralizing epitopes have the potential to provide sterilizing immunity by blocking initial mucosal infection.",

keywords = "Cytomegalovirus, Mucosal infection, Neutralizing antibody, Vaccine",

author = "Saccoccio, \{Frances M.\} and Sauer, \{Anne L.\} and Xiaohong Cui and Armstrong, \{Amy E.\} and Habib, \{EL Sayed E.\} and Johnson, \{David C.\} and Ryckman, \{Brent J.\} and Klingelhutz, \{Aloysius J.\} and Adler, \{Stuart P.\} and McVoy, \{Michael A.\}",

note = "Funding Information: We thank Ronzo Lee for technical assistance, Dai Wang and Thomas Shenk for providing BAD r UL131-Y4 BAC, David Gewirtz for use of the LX70 Inverted UV microscope, and Huiping Zhou for use of the Victor 3 V 1420 Multilable Counter. This work was supported in part by NIH/NIAID grant R21AI073615 (to MM), NIH/NIAID fellowship 1F31A073209 (to AS), and a Summer Research Fellowship Award from the Virginia Commonwealth University School of Medicine (to AA).",

year = "2011",

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doi = "10.1016/j.vaccine.2011.01.079",

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AU - Saccoccio, Frances M.

AU - Sauer, Anne L.

AU - Cui, Xiaohong

AU - Armstrong, Amy E.

AU - Habib, EL Sayed E.

AU - Johnson, David C.

AU - Ryckman, Brent J.

AU - Klingelhutz, Aloysius J.

AU - Adler, Stuart P.

AU - McVoy, Michael A.

N1 - Funding Information: We thank Ronzo Lee for technical assistance, Dai Wang and Thomas Shenk for providing BAD r UL131-Y4 BAC, David Gewirtz for use of the LX70 Inverted UV microscope, and Huiping Zhou for use of the Victor 3 V 1420 Multilable Counter. This work was supported in part by NIH/NIAID grant R21AI073615 (to MM), NIH/NIAID fellowship 1F31A073209 (to AS), and a Summer Research Fellowship Award from the Virginia Commonwealth University School of Medicine (to AA).

PY - 2011/3/24

Y1 - 2011/3/24

N2 - Cytomegalovirus infections are an important cause of disease for which no licensed vaccine exists. Recent studies have focused on the gH/gL/UL128-131 complex as antibodies to gH/gL/UL128-131 neutralize viral entry into epithelial cells. Prior studies have used cells from the retinal pigment epithelium, while to prevent transmission, vaccine-induced antibodies may need to block viral infection of epithelial cells of the oral or genital mucosa. We found that gH/gL/UL128-131 is necessary for efficient viral entry into epithelial cells derived from oral and genital mucosa, that short peptides from UL130 and UL131 elicit high titer neutralizing antibodies in rabbits, and that such antibodies neutralize viral entry into epithelial cells derived from these relevant tissues. These results suggest that single subunits or peptides may be sufficient to elicit potent epithelial entry neutralizing responses and that secretory antibodies to such neutralizing epitopes have the potential to provide sterilizing immunity by blocking initial mucosal infection.

AB - Cytomegalovirus infections are an important cause of disease for which no licensed vaccine exists. Recent studies have focused on the gH/gL/UL128-131 complex as antibodies to gH/gL/UL128-131 neutralize viral entry into epithelial cells. Prior studies have used cells from the retinal pigment epithelium, while to prevent transmission, vaccine-induced antibodies may need to block viral infection of epithelial cells of the oral or genital mucosa. We found that gH/gL/UL128-131 is necessary for efficient viral entry into epithelial cells derived from oral and genital mucosa, that short peptides from UL130 and UL131 elicit high titer neutralizing antibodies in rabbits, and that such antibodies neutralize viral entry into epithelial cells derived from these relevant tissues. These results suggest that single subunits or peptides may be sufficient to elicit potent epithelial entry neutralizing responses and that secretory antibodies to such neutralizing epitopes have the potential to provide sterilizing immunity by blocking initial mucosal infection.

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KW - Mucosal infection

KW - Neutralizing antibody

KW - Vaccine

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ER -

Peptides from cytomegalovirus UL130 and UL131 proteins induce high titer antibodies that block viral entry into mucosal epithelial cells

Abstract

Funding

Keywords

UN SDGs

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