TY - JOUR
T1 - Performance and selectivity of polymeric pseudostationary phases for the electrokinetic separation of amino acid derivatives and peptides
AU - Schulte, Shannon
AU - Singh, Anup K.
AU - Rauk, Erika
AU - Palmer, Christopher P.
PY - 2005/6
Y1 - 2005/6
N2 - Two polymeric pseudostationary phases, one an acrylamide polymer and the second a siloxane polymer, have been investigated for the separation of naphthalene-2,3-dicarboxaldehyde (NDA)-derivatized amino acids and small peptides. The dervatized amino acids were detected by UV absorbance and laser-induced fluorescence (LIF) detection. The polymers provided very high efficiency and good selectivity for the separation of the amino acids. The separation selectivity using the polymers was significantly different from that of SDS micelles, and there were subtle differences in selectivities between the polymers. Although very good detection limits were obtained with LIF detection, a significant background signal was observed when the polymers were not washed to remove fluorescent impurities. The polymers did not separate the peptides very well. It is postulated that the fixed covalent structure of the polymers prevents them from interacting strongly or efficiently with the peptides, which are large in relation to the analytes typically separated by electrokinetic chromatography using polymers.
AB - Two polymeric pseudostationary phases, one an acrylamide polymer and the second a siloxane polymer, have been investigated for the separation of naphthalene-2,3-dicarboxaldehyde (NDA)-derivatized amino acids and small peptides. The dervatized amino acids were detected by UV absorbance and laser-induced fluorescence (LIF) detection. The polymers provided very high efficiency and good selectivity for the separation of the amino acids. The separation selectivity using the polymers was significantly different from that of SDS micelles, and there were subtle differences in selectivities between the polymers. Although very good detection limits were obtained with LIF detection, a significant background signal was observed when the polymers were not washed to remove fluorescent impurities. The polymers did not separate the peptides very well. It is postulated that the fixed covalent structure of the polymers prevents them from interacting strongly or efficiently with the peptides, which are large in relation to the analytes typically separated by electrokinetic chromatography using polymers.
KW - Amino acids
KW - Electrokinetic chromatography
KW - Peptides
KW - Polymer
KW - Pseudosstationary phase
UR - http://www.scopus.com/inward/record.url?scp=21244502174&partnerID=8YFLogxK
U2 - 10.1007/s00216-005-3131-6
DO - 10.1007/s00216-005-3131-6
M3 - Article
C2 - 15933853
AN - SCOPUS:21244502174
SN - 1618-2642
VL - 382
SP - 777
EP - 782
JO - Analytical and Bioanalytical Chemistry
JF - Analytical and Bioanalytical Chemistry
IS - 3
ER -