Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase

Annie C.Y. Chang; Jack H. Nunberg; Randal J. Kaufman; Henry A. Erlich; Robert T. Schimke; Stanley N. Cohen

doi:10.1038/275617a0

Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase

Annie C.Y. Chang, Jack H. Nunberg, Randal J. Kaufman, Henry A. Erlich, Robert T. Schimke, Stanley N. Cohen

Montana Biotechnology Center

Stanford University

Research output: Contribution to journal › Article › peer-review

238 Scopus citations

Abstract

The construction and analysis of bacterial plasmids that contain and phenotypically express a mammalian genetic sequence are described. Such plasmids specify a protein that has enzymatic properties, immunological reactivity and molecular size characteristic of the mouse dihydrofolate reductase, and render host cells resistant to the anti-metabolic drug trimethoprim.

Original language	English
Pages (from-to)	617-624
Number of pages	8
Journal	Nature
Volume	275
Issue number	5681
DOIs	https://doi.org/10.1038/275617a0
State	Published - 1978

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@article{0b6b6a0cbe634e1d85437ae19462f4bf,

title = "Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase",

abstract = "The construction and analysis of bacterial plasmids that contain and phenotypically express a mammalian genetic sequence are described. Such plasmids specify a protein that has enzymatic properties, immunological reactivity and molecular size characteristic of the mouse dihydrofolate reductase, and render host cells resistant to the anti-metabolic drug trimethoprim.",

author = "Chang, {Annie C.Y.} and Nunberg, {Jack H.} and Kaufman, {Randal J.} and Erlich, {Henry A.} and Schimke, {Robert T.} and Cohen, {Stanley N.}",

year = "1978",

doi = "10.1038/275617a0",

language = "English",

volume = "275",

pages = "617--624",

journal = "Nature",

issn = "0028-0836",

publisher = "Nature Research",

number = "5681",

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T1 - Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase

AU - Chang, Annie C.Y.

AU - Nunberg, Jack H.

AU - Kaufman, Randal J.

AU - Erlich, Henry A.

AU - Schimke, Robert T.

AU - Cohen, Stanley N.

PY - 1978

Y1 - 1978

N2 - The construction and analysis of bacterial plasmids that contain and phenotypically express a mammalian genetic sequence are described. Such plasmids specify a protein that has enzymatic properties, immunological reactivity and molecular size characteristic of the mouse dihydrofolate reductase, and render host cells resistant to the anti-metabolic drug trimethoprim.

AB - The construction and analysis of bacterial plasmids that contain and phenotypically express a mammalian genetic sequence are described. Such plasmids specify a protein that has enzymatic properties, immunological reactivity and molecular size characteristic of the mouse dihydrofolate reductase, and render host cells resistant to the anti-metabolic drug trimethoprim.

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U2 - 10.1038/275617a0

DO - 10.1038/275617a0

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C2 - 360074

AN - SCOPUS:0018077902

SN - 0028-0836

VL - 275

SP - 617

EP - 624

JO - Nature

JF - Nature

IS - 5681

ER -

Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase

Abstract

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