Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase

Annie C.Y. Chang, Jack H. Nunberg, Randal J. Kaufman, Henry A. Erlich, Robert T. Schimke, Stanley N. Cohen

Research output: Contribution to journalArticlepeer-review

Abstract

The construction and analysis of bacterial plasmids that contain and phenotypically express a mammalian genetic sequence are described. Such plasmids specify a protein that has enzymatic properties, immunological reactivity and molecular size characteristic of the mouse dihydrofolate reductase, and render host cells resistant to the anti-metabolic drug trimethoprim.

Original languageEnglish
Pages (from-to)617-624
Number of pages8
JournalNature
Volume275
Issue number5681
DOIs
StatePublished - 1978

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