Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase

Annie C.Y. Chang; Jack H. Nunberg; Randal J. Kaufman; Henry A. Erlich; Robert T. Schimke; Stanley N. Cohen

doi:10.1038/275617a0

Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase

Annie C.Y. Chang
, Jack H. Nunberg
, Randal J. Kaufman
, Henry A. Erlich
, Robert T. Schimke
, Stanley N. Cohen

Montana Biotechnology Center

Stanford University

Research output: Contribution to journal › Article › peer-review

239 Scopus citations

Abstract

The construction and analysis of bacterial plasmids that contain and phenotypically express a mammalian genetic sequence are described. Such plasmids specify a protein that has enzymatic properties, immunological reactivity and molecular size characteristic of the mouse dihydrofolate reductase, and render host cells resistant to the anti-metabolic drug trimethoprim.

Original language	English
Pages (from-to)	617-624
Number of pages	8
Journal	Nature
Volume	275
Issue number	5681
DOIs	https://doi.org/10.1038/275617a0
State	Published - 1978

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@article{0b6b6a0cbe634e1d85437ae19462f4bf,

title = "Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase",

abstract = "The construction and analysis of bacterial plasmids that contain and phenotypically express a mammalian genetic sequence are described. Such plasmids specify a protein that has enzymatic properties, immunological reactivity and molecular size characteristic of the mouse dihydrofolate reductase, and render host cells resistant to the anti-metabolic drug trimethoprim.",

author = "Chang, \{Annie C.Y.\} and Nunberg, \{Jack H.\} and Kaufman, \{Randal J.\} and Erlich, \{Henry A.\} and Schimke, \{Robert T.\} and Cohen, \{Stanley N.\}",

year = "1978",

doi = "10.1038/275617a0",

language = "English",

volume = "275",

pages = "617--624",

journal = "Nature",

issn = "0028-0836",

publisher = "Nature Research",

number = "5681",

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T1 - Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase

AU - Chang, Annie C.Y.

AU - Nunberg, Jack H.

AU - Kaufman, Randal J.

AU - Erlich, Henry A.

AU - Schimke, Robert T.

AU - Cohen, Stanley N.

PY - 1978

Y1 - 1978

N2 - The construction and analysis of bacterial plasmids that contain and phenotypically express a mammalian genetic sequence are described. Such plasmids specify a protein that has enzymatic properties, immunological reactivity and molecular size characteristic of the mouse dihydrofolate reductase, and render host cells resistant to the anti-metabolic drug trimethoprim.

AB - The construction and analysis of bacterial plasmids that contain and phenotypically express a mammalian genetic sequence are described. Such plasmids specify a protein that has enzymatic properties, immunological reactivity and molecular size characteristic of the mouse dihydrofolate reductase, and render host cells resistant to the anti-metabolic drug trimethoprim.

UR - https://www.scopus.com/pages/publications/0018077902

U2 - 10.1038/275617a0

DO - 10.1038/275617a0

M3 - Article

C2 - 360074

AN - SCOPUS:0018077902

SN - 0028-0836

VL - 275

SP - 617

EP - 624

JO - Nature

JF - Nature

IS - 5681

ER -

Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase

Abstract

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