TY - JOUR
T1 - Phospholipase A2 antagonists inhibit constitutive retrograde membrane traffic to the reticulum
AU - De Figueiredo, Paul
AU - Drecktrah, Dan
AU - Polizotto, Renee S.
AU - Cole, Nelson B.
AU - Lippincott-Schwartz, Jennifer
AU - Brown, William J.
PY - 2000
Y1 - 2000
N2 - Eukaryotic cells contain a variety of cytoplasmic Ca2+ -dependent and Ca2+ -independent phospholipase A2s (PLA2s; EC 2.3.1.2.3). However, the physiological roles for many of these ubiquitously-expressed enzymes is unclear or not known. Recently, pharmacological studies have suggested a role for Ca2+ -independent PLA2 (iPLA2) enzymes in governing intracellular membrane trafficking events in general and regulating brefeldin A (BFA)-stimulated membrane tubulation and Golgi-to-endoplasmic reticulum (ER) retrograde membrane trafficking, in particular. Here, we extend these studies to show that membrane-permeant iPLA2 antagonists potently inhibit the normal, constitutive retrograde membrane trafficking from the trans-Golgi network 〈TGN〉, Golgi complex, and the ERGIC-53-positive ER-Golgi-intermediate compartment (ERGIC), which occurs in the absence of BFA. Taken together, these results suggest that iPLA2 enzymes play a general role in regulating, or directly mediating, multiple mammalian membrane trafficking events.
AB - Eukaryotic cells contain a variety of cytoplasmic Ca2+ -dependent and Ca2+ -independent phospholipase A2s (PLA2s; EC 2.3.1.2.3). However, the physiological roles for many of these ubiquitously-expressed enzymes is unclear or not known. Recently, pharmacological studies have suggested a role for Ca2+ -independent PLA2 (iPLA2) enzymes in governing intracellular membrane trafficking events in general and regulating brefeldin A (BFA)-stimulated membrane tubulation and Golgi-to-endoplasmic reticulum (ER) retrograde membrane trafficking, in particular. Here, we extend these studies to show that membrane-permeant iPLA2 antagonists potently inhibit the normal, constitutive retrograde membrane trafficking from the trans-Golgi network 〈TGN〉, Golgi complex, and the ERGIC-53-positive ER-Golgi-intermediate compartment (ERGIC), which occurs in the absence of BFA. Taken together, these results suggest that iPLA2 enzymes play a general role in regulating, or directly mediating, multiple mammalian membrane trafficking events.
KW - Golgi complex
KW - Membrane tubules
KW - Phospholipase A
KW - Retrograde transport
UR - http://www.scopus.com/inward/record.url?scp=0034208173&partnerID=8YFLogxK
U2 - 10.1034/j.1600-0854.2000.010608.x
DO - 10.1034/j.1600-0854.2000.010608.x
M3 - Article
C2 - 11208136
AN - SCOPUS:0034208173
SN - 1398-9219
VL - 1
SP - 504
EP - 511
JO - Traffic
JF - Traffic
IS - 6
ER -