Phospholipase A2 antagonists inhibit constitutive retrograde membrane traffic to the reticulum

Paul De Figueiredo, Dan Drecktrah, Renee S. Polizotto, Nelson B. Cole, Jennifer Lippincott-Schwartz, William J. Brown

Research output: Contribution to journalArticlepeer-review

57 Scopus citations


Eukaryotic cells contain a variety of cytoplasmic Ca2+ -dependent and Ca2+ -independent phospholipase A2s (PLA2s; EC However, the physiological roles for many of these ubiquitously-expressed enzymes is unclear or not known. Recently, pharmacological studies have suggested a role for Ca2+ -independent PLA2 (iPLA2) enzymes in governing intracellular membrane trafficking events in general and regulating brefeldin A (BFA)-stimulated membrane tubulation and Golgi-to-endoplasmic reticulum (ER) retrograde membrane trafficking, in particular. Here, we extend these studies to show that membrane-permeant iPLA2 antagonists potently inhibit the normal, constitutive retrograde membrane trafficking from the trans-Golgi network 〈TGN〉, Golgi complex, and the ERGIC-53-positive ER-Golgi-intermediate compartment (ERGIC), which occurs in the absence of BFA. Taken together, these results suggest that iPLA2 enzymes play a general role in regulating, or directly mediating, multiple mammalian membrane trafficking events.

Original languageEnglish
Pages (from-to)504-511
Number of pages8
Issue number6
StatePublished - 2000


  • Golgi complex
  • Membrane tubules
  • Phospholipase A
  • Retrograde transport


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