Polyamines and linear DNA mediate bacterial threat assessment of bacteriophage infection

Camilla D. de Mattos, Dominick R. Faith, Artem A. Nemudryi, Amelia K. Schmidt, De Anna C. Bublitz, Lauren Hammond, Margie A. Kinnersley, Caleb M. Schwartzkopf, Autumn J. Robinson, Alex Joyce, Lia A. Michaels, Robert S. Brzozowski, Alison Coluccio, Denghui David Xing, Jumpei Uchiyama, Laura K. Jennings, Prahathees Eswara, Blake Wiedenheft, Patrick R. Secor

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Monitoring the extracellular environment for danger signals is a critical aspect of cellular survival. However, the danger signals released by dying bacteria and the mechanisms bacteria use for threat assessment remain largely unexplored. Here, we show that lysis of Pseudomonas aeruginosa cells releases polyamines that are subsequently taken up by surviving cells via a mechanism that relies on Gac/Rsm signaling. While intracellular polyamines spike in surviving cells, the duration of this spike varies according to the infection status of the cell. In bacteriophage-infected cells, intracellular polyamines are maintained at high levels, which inhibits replication of the bacteriophage genome. Many bacteriophages package linear DNA genomes and linear DNA is sufficient to trigger intracellular polyamine accumulation, suggesting that linear DNA is sensed as a second danger signal. Collectively, these results demonstrate how polyamines released by dying cells together with linear DNA allow P. aeruginosa to make threat assessments of cellular injury.

Original languageEnglish
Article numbere2216430120
Pages (from-to)e2216430120
JournalProceedings of the National Academy of Sciences of the United States of America
Volume120
Issue number9
DOIs
StatePublished - Feb 28 2023

Keywords

  • Pseudomonas aeruginosa
  • bacteriophage
  • danger sensing
  • phage defense
  • polyamine
  • DNA
  • Bacteria
  • Polyamines
  • Bacteriophages/genetics

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