Power of 22 microsatellite markers in fluorescent multiplexes for parentage testing in goats (Capra hircus)

G. Luikart, M. P. Biju-Duval, O. Ertugrul, Y. Zagdsuren, C. Maudet, P. Taberlet

Research output: Contribution to journalArticlepeer-review

Abstract

Two multiplex systems, each containing 11 microsatellite loci, were developed for semi-automated parentage testing in goats. Eight of the loci originate from goats, nine from cattle and five from sheep. Eighteen of the loci have been mapped to 16 different autosomes (in goats and cattle). Parentage exclusion probabilities were computed from allele frequencies in ~30 unrelated individuals from each of four economically important breeds: Mongolian Native Cashmere, Turkish Angora, Swiss Saanen, and Spanish Murciana-Grenadina. In cases where genotypes are known for one parent and an offspring, the 22 markers will exclude an (erroneously) alleged parent with a probability of > 0.999999 in the cashmere breed, > 0.99999 in Angora and Murciana-Grenadina, and > 0.9999 in Saanen. The multiplexes provide very high power for individual identification as the probability of finding two identical genotypes for the 22 loci is < 1 in 1.1015 in each of the four breeds. The multiplexes will also be useful for studies of population structure, history, and diversity in goats and also in wild Capra species that represent important resources for genetic improvement of domestic breeds.

Original languageEnglish
Pages (from-to)431-438
Number of pages8
JournalAnimal Genetics
Volume30
Issue number6
DOIs
StatePublished - Dec 1999

Keywords

  • Exclusion probabilities
  • Individual identification
  • PCR amplification
  • Paternity testing

Fingerprint

Dive into the research topics of 'Power of 22 microsatellite markers in fluorescent multiplexes for parentage testing in goats (Capra hircus)'. Together they form a unique fingerprint.

Cite this