TY - JOUR
T1 - Protein interactions regulating vesicle transport between the endoplasmic reticulum and Golgi apparatus in mammalian cells
AU - Hay, Jesse C.
AU - Chao, Daniel S.
AU - Kuo, Christin S.
AU - Scheller, Richard H.
PY - 1997/4/4
Y1 - 1997/4/4
N2 - The proposed cis-Golgi vesicle receptor syntaxin 5 was found in a complex with Golgi-associated SNARE of 28 kDa (GOS-28), rbet1, rsly1, and two novel proteins characterized herein: rat sec22b and membrin, both cytoplasmically oriented integral membrane proteins. The complex appears to recapitulate vesicle docking interactions of proteins originating from distinct compartments, since syntaxin 5, rbet1, and GOS-28 localize to Golgi membranes, whereas mouse sec22b and membrin accumulate in the endoplasmic reticulum. Protein interactions in the complex are dramatically rearranged by N-ethylmaleimide-sensitive factor. The complex consists of two or more subcomplexes with some members (rat sec22b and syntaxin 5) in common and others (rbet1 and GOS-28) mutually exclusively associated. We propose that these protein interactions determine vesicle docking/fusion fidelity between the endoplasmic reticulum and Golgi.
AB - The proposed cis-Golgi vesicle receptor syntaxin 5 was found in a complex with Golgi-associated SNARE of 28 kDa (GOS-28), rbet1, rsly1, and two novel proteins characterized herein: rat sec22b and membrin, both cytoplasmically oriented integral membrane proteins. The complex appears to recapitulate vesicle docking interactions of proteins originating from distinct compartments, since syntaxin 5, rbet1, and GOS-28 localize to Golgi membranes, whereas mouse sec22b and membrin accumulate in the endoplasmic reticulum. Protein interactions in the complex are dramatically rearranged by N-ethylmaleimide-sensitive factor. The complex consists of two or more subcomplexes with some members (rat sec22b and syntaxin 5) in common and others (rbet1 and GOS-28) mutually exclusively associated. We propose that these protein interactions determine vesicle docking/fusion fidelity between the endoplasmic reticulum and Golgi.
UR - http://www.scopus.com/inward/record.url?scp=0030890594&partnerID=8YFLogxK
U2 - 10.1016/S0092-8674(00)80191-9
DO - 10.1016/S0092-8674(00)80191-9
M3 - Article
C2 - 9094723
AN - SCOPUS:0030890594
SN - 0092-8674
VL - 89
SP - 149
EP - 158
JO - Cell
JF - Cell
IS - 1
ER -