Pseudomonas phage inhibition of Candida albicans

Hasan Nazik, Lydia Marie Joubert, Patrick R. Secor, Johanna M. Sweere, Paul L. Bollyky, Gabriele Sass, Lynette Cegelski, David A. Stevens

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


Pseudomonas aeruginosa (Pa) and Candida albicans (Ca) are major bacterial and fungal pathogens in immunocompromised hosts, and notably in the airways of cystic fibrosis patients. The bacteriophages of Pa physically alter biofilms, and were recently shown to inhibit the biofilms of Aspergillus fumigatus. To understand the range of this viral-fungal interaction, we studied Pa phages Pf4 and Pf1, and their interactions with Ca biofilm formation and preformed Ca biofilm. Both forms of Ca biofilm development, as well as planktonic Ca growth, were inhibited by either phage. The inhibition of biofilm was reversed by the addition of iron, suggesting that the mechanism of phage action on Ca involves denial of iron. Birefringence studies on added phage showed an ordered structure of binding to Ca. Electron microscopic observations indicated phage aggregation in the biofilm extracellular matrix. Bacteriophage-fungal interactions may be a general feature with several pathogens in the fungal kingdom.

Original languageEnglish
Article number000539
Pages (from-to)1568-1577
Number of pages10
Issue number11
StatePublished - Nov 2017


  • Bacteriophage
  • Biofilm
  • Candida albicans
  • Iron
  • Pseudomonas aeruginosa


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