RAD sequencing yields a high success rate for westslope cutthroat and rainbow trout species-diagnostic SNP assays

Stephen J. Amish, Paul A. Hohenlohe, Sally Painter, Robb F. Leary, Clint Muhlfeld, Fred W. Allendorf, Gordon Luikart

Research output: Contribution to journalArticlepeer-review

Abstract

Hybridization with introduced rainbow trout threatens most native westslope cutthroat trout populations. Understanding the genetic effects of hybridization and introgression requires a large set of high-throughput, diagnostic genetic markers to inform conservation and management. Recently, we identified several thousand candidate single-nucleotide polymorphism (SNP) markers based on RAD sequencing of 11 westslope cutthroat trout and 13 rainbow trout individuals. Here, we used flanking sequence for 56 of these candidate SNP markers to design high-throughput genotyping assays. We validated the assays on a total of 92 individuals from 22 populations and seven hatchery strains. Forty-six assays (82%) amplified consistently and allowed easy identification of westslope cutthroat and rainbow trout alleles as well as heterozygote controls. The 46 SNPs will provide high power for early detection of population admixture and improved identification of hybrid and nonhybridized individuals. This technique shows promise as a very low-cost, reliable and relatively rapid method for developing and testing SNP markers for nonmodel organisms with limited genomic resources.

Original languageEnglish
Pages (from-to)653-660
Number of pages8
JournalMolecular Ecology Resources
Volume12
Issue number4
DOIs
StatePublished - Jul 2012

Keywords

  • Conservation genomics
  • Hybridization
  • Introgression
  • Invasive species
  • Microfluidic PCR
  • SNP
  • Salmonids
  • Trout species identification

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