Rapid quantification of cardiolipin and DOPC lipid and vesicle concentration

Margaret M. Elmer-Dixon; Bruce E. Bowler

doi:10.1016/j.ab.2016.12.024

Rapid quantification of cardiolipin and DOPC lipid and vesicle concentration

Margaret M. Elmer-Dixon
, Bruce E. Bowler

Chemistry and Biochemistry

University of Montana

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

A novel approach to quantification of cardiolipin and DOPC lipid and vesicle concentration that is rapid and inexpensive is described. Traditional approaches to quantifying vesicle concentration destroy sample and are often time consuming. Using common laboratory equipment and software, lipid vesicles were reliably quantified allowing for immediate use without significant sample loss. Once calibrated, only absorbance measurements with a UV-Vis spectrophotometer are necessary as input into a Matlab program, which calculates the corresponding vesicle and lipid concentration. Fast and accurate concentration determination for preparations of vesicles is essential for analytical titration experiments necessary for protein/vesicle binding curves.

Original language	English
Pages (from-to)	58-61
Number of pages	4
Journal	Analytical Biochemistry
Volume	520
DOIs	https://doi.org/10.1016/j.ab.2016.12.024
State	Published - Mar 1 2017

Funding

We thank Andre Umansky in Environmental Biogeochemistry Laboratory at the University of Montana for assistance with ICP measurements. Funding: This work was supported by the National Science Foundation [grant numbers: CHE-1306903 and CHE-1609720] to B.E.B.

Funder number
CHE-1609720, CHE-1306903

Keywords

Cardiolipin
DOPC
Lipid quantification
POPC
POPG
Vesicle quantification

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10.1016/j.ab.2016.12.024

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abstract = "A novel approach to quantification of cardiolipin and DOPC lipid and vesicle concentration that is rapid and inexpensive is described. Traditional approaches to quantifying vesicle concentration destroy sample and are often time consuming. Using common laboratory equipment and software, lipid vesicles were reliably quantified allowing for immediate use without significant sample loss. Once calibrated, only absorbance measurements with a UV-Vis spectrophotometer are necessary as input into a Matlab program, which calculates the corresponding vesicle and lipid concentration. Fast and accurate concentration determination for preparations of vesicles is essential for analytical titration experiments necessary for protein/vesicle binding curves.",

keywords = "Cardiolipin, DOPC, Lipid quantification, POPC, POPG, Vesicle quantification",

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AU - Elmer-Dixon, Margaret M.

AU - Bowler, Bruce E.

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N2 - A novel approach to quantification of cardiolipin and DOPC lipid and vesicle concentration that is rapid and inexpensive is described. Traditional approaches to quantifying vesicle concentration destroy sample and are often time consuming. Using common laboratory equipment and software, lipid vesicles were reliably quantified allowing for immediate use without significant sample loss. Once calibrated, only absorbance measurements with a UV-Vis spectrophotometer are necessary as input into a Matlab program, which calculates the corresponding vesicle and lipid concentration. Fast and accurate concentration determination for preparations of vesicles is essential for analytical titration experiments necessary for protein/vesicle binding curves.

AB - A novel approach to quantification of cardiolipin and DOPC lipid and vesicle concentration that is rapid and inexpensive is described. Traditional approaches to quantifying vesicle concentration destroy sample and are often time consuming. Using common laboratory equipment and software, lipid vesicles were reliably quantified allowing for immediate use without significant sample loss. Once calibrated, only absorbance measurements with a UV-Vis spectrophotometer are necessary as input into a Matlab program, which calculates the corresponding vesicle and lipid concentration. Fast and accurate concentration determination for preparations of vesicles is essential for analytical titration experiments necessary for protein/vesicle binding curves.

KW - Cardiolipin

KW - DOPC

KW - Lipid quantification

KW - POPC

KW - POPG

KW - Vesicle quantification

UR - https://www.scopus.com/pages/publications/85009195256

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DO - 10.1016/j.ab.2016.12.024

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VL - 520

SP - 58

EP - 61

JO - Analytical Biochemistry

JF - Analytical Biochemistry

ER -

Rapid quantification of cardiolipin and DOPC lipid and vesicle concentration

Abstract

Funding

Keywords

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