Rapid quantification of vesicle concentration for DOPG/DOPC and Cardiolipin/DOPC mixed lipid systems of variable composition

Margaret M. Elmer-Dixon, Bruce E. Bowler

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

A novel approach to quantify mixed lipid systems is described. Traditional approaches to lipid vesicle quantification are time consuming, require large amounts of material and are destructive. We extend our recently described method for quantification of pure lipid systems to mixed lipid systems. The method only requires a UV–Vis spectrometer and does not destroy sample. Mie scattering data from absorbance measurements are used as input into a Matlab program to calculate the total vesicle concentration and the concentrations of each lipid in the mixed lipid system. The technique is fast and accurate, which is essential for analytical lipid binding experiments.

Original languageEnglish
Pages (from-to)12-14
Number of pages3
JournalAnalytical Biochemistry
Volume553
DOIs
StatePublished - Jul 15 2018

Keywords

  • DOPC
  • DOPG
  • Liposome quantification
  • Mixed lipid quantification
  • TOCL
  • Vesicle quantification

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