Rapid quantification of vesicle concentration for DOPG/DOPC and Cardiolipin/DOPC mixed lipid systems of variable composition

Margaret M. Elmer-Dixon; Bruce E. Bowler

doi:10.1016/j.ab.2018.05.013

Rapid quantification of vesicle concentration for DOPG/DOPC and Cardiolipin/DOPC mixed lipid systems of variable composition

Margaret M. Elmer-Dixon
, Bruce E. Bowler

Chemistry and Biochemistry

University of Montana

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A novel approach to quantify mixed lipid systems is described. Traditional approaches to lipid vesicle quantification are time consuming, require large amounts of material and are destructive. We extend our recently described method for quantification of pure lipid systems to mixed lipid systems. The method only requires a UV–Vis spectrometer and does not destroy sample. Mie scattering data from absorbance measurements are used as input into a Matlab program to calculate the total vesicle concentration and the concentrations of each lipid in the mixed lipid system. The technique is fast and accurate, which is essential for analytical lipid binding experiments.

Original language	English
Pages (from-to)	12-14
Number of pages	3
Journal	Analytical Biochemistry
Volume	553
DOIs	https://doi.org/10.1016/j.ab.2018.05.013
State	Published - Jul 15 2018

Funding

We thank Andre Umansky and Matt Young in the Environmental Biogeochemistry Laboratory at the University of Montana for assistance with ICP measurements. Funding: This work was supported by the National Science Foundation [grant numbers: CHE-1306903 and CHE-1609720] to B.E.B.

Funder number
CHE-1609720, CHE-1306903

Keywords

DOPC
DOPG
Liposome quantification
Mixed lipid quantification
TOCL
Vesicle quantification

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10.1016/j.ab.2018.05.013

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title = "Rapid quantification of vesicle concentration for DOPG/DOPC and Cardiolipin/DOPC mixed lipid systems of variable composition",

abstract = "A novel approach to quantify mixed lipid systems is described. Traditional approaches to lipid vesicle quantification are time consuming, require large amounts of material and are destructive. We extend our recently described method for quantification of pure lipid systems to mixed lipid systems. The method only requires a UV–Vis spectrometer and does not destroy sample. Mie scattering data from absorbance measurements are used as input into a Matlab program to calculate the total vesicle concentration and the concentrations of each lipid in the mixed lipid system. The technique is fast and accurate, which is essential for analytical lipid binding experiments.",

keywords = "DOPC, DOPG, Liposome quantification, Mixed lipid quantification, TOCL, Vesicle quantification",

author = "Elmer-Dixon, \{Margaret M.\} and Bowler, \{Bruce E.\}",

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doi = "10.1016/j.ab.2018.05.013",

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T1 - Rapid quantification of vesicle concentration for DOPG/DOPC and Cardiolipin/DOPC mixed lipid systems of variable composition

AU - Elmer-Dixon, Margaret M.

AU - Bowler, Bruce E.

PY - 2018/7/15

Y1 - 2018/7/15

N2 - A novel approach to quantify mixed lipid systems is described. Traditional approaches to lipid vesicle quantification are time consuming, require large amounts of material and are destructive. We extend our recently described method for quantification of pure lipid systems to mixed lipid systems. The method only requires a UV–Vis spectrometer and does not destroy sample. Mie scattering data from absorbance measurements are used as input into a Matlab program to calculate the total vesicle concentration and the concentrations of each lipid in the mixed lipid system. The technique is fast and accurate, which is essential for analytical lipid binding experiments.

AB - A novel approach to quantify mixed lipid systems is described. Traditional approaches to lipid vesicle quantification are time consuming, require large amounts of material and are destructive. We extend our recently described method for quantification of pure lipid systems to mixed lipid systems. The method only requires a UV–Vis spectrometer and does not destroy sample. Mie scattering data from absorbance measurements are used as input into a Matlab program to calculate the total vesicle concentration and the concentrations of each lipid in the mixed lipid system. The technique is fast and accurate, which is essential for analytical lipid binding experiments.

KW - DOPC

KW - DOPG

KW - Liposome quantification

KW - Mixed lipid quantification

KW - TOCL

KW - Vesicle quantification

UR - https://www.scopus.com/pages/publications/85047065943

U2 - 10.1016/j.ab.2018.05.013

DO - 10.1016/j.ab.2018.05.013

M3 - Article

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AN - SCOPUS:85047065943

SN - 0003-2697

VL - 553

SP - 12

EP - 14

JO - Analytical Biochemistry

JF - Analytical Biochemistry

ER -

Rapid quantification of vesicle concentration for DOPG/DOPC and Cardiolipin/DOPC mixed lipid systems of variable composition

Abstract

Funding

Keywords

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