Reconstitution of COPII vesicle fusion to generate a pre-Golgi intermediate compartment

Dalu Xu, Jesse C. Hay

Research output: Contribution to journalArticlepeer-review

84 Scopus citations


What is the first membrane fusion step in the secretory pathway? In mammals, transport vesicles coated with coat complex (COP) II deliver secretory cargo to vesicular tubular clusters (VTCs) that ferry cargo from endoplasmic reticulum exit sites to the Golgi stack. However, the precise origin of VTCs and the membrane fusion step(s) involved have remained experimentally intractable. Here, we document in vitro direct tethering and SNARE-dependent fusion of endoplasmic reticulum-derived COPII transport vesicles to form larger cargo containers. The assembly did not require detectable Golgi membranes, preexisting VTCs, or COPI function. Therefore, COPII vesicles appear to contain all of the machinery to initiate VTC biogenesis via homotypic fusion. However, COPI function enhanced VTC assembly, and early VTCs acquired specific Golgi components by heterotypic fusion with Golgi-derived COPI vesicles.

Original languageEnglish
Pages (from-to)997-1003
Number of pages7
JournalJournal of Cell Biology
Issue number6
StatePublished - Dec 20 2004


Dive into the research topics of 'Reconstitution of COPII vesicle fusion to generate a pre-Golgi intermediate compartment'. Together they form a unique fingerprint.

Cite this