Reduced Representation Methods for Subgenomic Enrichment and Next-Generation Sequencing

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27 Scopus citations

Abstract

Several methods have been developed to enrich DNA for subsets of the genome prior to next-generation sequencing. These front-end enrichment strategies provide powerful and cost-effective tools for researchers interested in collecting large-scale genomic sequence data. In this review, I provide an overview of both general and targeted reduced representation enrichment strategies that are commonly used in tandem with next-generation sequencing. I focus on several key issues that are likely to be important when deciding which enrichment strategy is most appropriate for a given experiment. Overall, these techniques can enable the collection of large-scale genomic data in diverse species, providing a powerful tool for the study of evolutionary biology.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages85-103
Number of pages19
DOIs
StatePublished - 2011

Publication series

NameMethods in Molecular Biology
Volume772
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Ancient DNA
  • Reduced representation
  • Sample bar coding
  • Sequence capture
  • Targeted resequencing

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