Sequencing primers and SNPs for rapidly evolving reproductive loci in endangered ibex and their kin (Bovidae, Capra spp.)

Steve Jordan, Christian Miquel, Pierre Taberlet, Gordon Luikart

Research output: Contribution to journalArticlepeer-review

Abstract

Rapidly evolving genes (e.g. candidate selected loci) are of increasing interest to molecular ecologists and conservation geneticists. Here, we report primers for five regions from three independent nuclear reproductive genes that reliably generate polymorphic sequences across the widespread wild goats of the Capra ibex species group and likely many other species of bovids. From three to nine single-nucleotide polymorphisms (SNPs) were identified in each gene region among C. ibex subspecies. Average numbers of SNPs per 1000 bp across all five gene regions was 15.0, with a high of 25.3 in the ZP3 exons 3 and 4 sequence and a low of 6.1 in the TNP1 sequence.

Original languageEnglish
Pages (from-to)776-779
Number of pages4
JournalMolecular Ecology Notes
Volume6
Issue number3
DOIs
StatePublished - Sep 2006

Keywords

  • Candidate genes
  • Genomics
  • Molecular adaptation
  • Phylogenomics
  • Phylogeny
  • SNPs
  • Selection

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