Skeletal muscle metabolic gene response to carbohydrate feeding during exercise in the heat

Charles L. Dumke; Dustin R. Slivka; John S. Cuddy; Walter S. Hailes; Brent C. Ruby

doi:10.1186/1550-2783-10-40

Skeletal muscle metabolic gene response to carbohydrate feeding during exercise in the heat

Charles L. Dumke, Dustin R. Slivka, John S. Cuddy, Walter S. Hailes, Brent C. Ruby

Integrative Physiology and Athletic Training

University of Montana

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Background: Heat stress down-regulates mitochondrial function, while carbohydrate supplementation attenuates the exercise induced stimulation of mitochondrial biogenesis in humans. The effects of exogenous carbohydrate during exercise in the heat on metabolic mRNA have not been investigated in humans. The purpose of this study was to determine the impact of exercise with and without carbohydrate supplementation on skeletal muscle metabolic response in the heat. Methods: Eight recreationally active males (4.05 ± 0.2 L^.min^-1) completed 2 trials which included 1 hr of cycling at 70% workload max and 3 hr recovery in a hot environment. Both trials were conducted in a climate controlled environmental chamber (38°C and 40% RH). The trials differed by the consumption of either a 6% carbohydrate (CHO) containing beverage (8 ml^.kg^-1.hr^-1) or placebo (P) during exercise in random order. Muscle biopsies were obtained from the vastus lateralis before exercise, immediately post-exercise and at the end of the 3 hr recovery period. Muscle was analyzed for muscle glycogen and mRNA related to metabolic and mitochondrial development (MFN2, PGC-1α, GLUT4, UCP3). Expired gases were measured to determine whole body substrate use during exercise. Results: Carbohydrate oxidation and muscle glycogen utilization did not differ between trials, whereas fat oxidation was elevated during exercise in P. Exercise caused an increase in PGC-1α, and GLUT4 (P < 0.05) independent of exogenous carbohydrate provision. Carbohydrate consumption attenuated the mRNA response in UCP3 (P < 0.05). Conclusions: This study indicates that the provision of exogenous carbohydrate attenuates the stimulation of mRNA expression of UCP3 following exercise in the heat.

Original language	English
Article number	40
Journal	Journal of the International Society of Sports Nutrition
Volume	10
DOIs	https://doi.org/10.1186/1550-2783-10-40
State	Published - Sep 13 2013

Funding

The authors declare that they have no competing interests in access to these data or associations with companies involved with products used in this research. This study was supported by a grant from the Office of Naval Research Grant Award N000140910850. The views and conclusions contained herein are those of the authors and should not be interpreted as necessarily representing the official policies or endorsements, either expressed or implied, of the Office of Naval Research or the U.S. government.

Funders	Funder number
Office of Naval Research	N000140910850

Keywords

Carbohydrate supplementation
Mitochondrial biogenesis
PGC-1α
Substrate utilization
UCP3

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1186/1550-2783-10-40

Cite this

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title = "Skeletal muscle metabolic gene response to carbohydrate feeding during exercise in the heat",

abstract = "Background: Heat stress down-regulates mitochondrial function, while carbohydrate supplementation attenuates the exercise induced stimulation of mitochondrial biogenesis in humans. The effects of exogenous carbohydrate during exercise in the heat on metabolic mRNA have not been investigated in humans. The purpose of this study was to determine the impact of exercise with and without carbohydrate supplementation on skeletal muscle metabolic response in the heat. Methods: Eight recreationally active males (4.05 ± 0.2 L.min-1) completed 2 trials which included 1 hr of cycling at 70% workload max and 3 hr recovery in a hot environment. Both trials were conducted in a climate controlled environmental chamber (38°C and 40% RH). The trials differed by the consumption of either a 6% carbohydrate (CHO) containing beverage (8 ml.kg-1.hr-1) or placebo (P) during exercise in random order. Muscle biopsies were obtained from the vastus lateralis before exercise, immediately post-exercise and at the end of the 3 hr recovery period. Muscle was analyzed for muscle glycogen and mRNA related to metabolic and mitochondrial development (MFN2, PGC-1α, GLUT4, UCP3). Expired gases were measured to determine whole body substrate use during exercise. Results: Carbohydrate oxidation and muscle glycogen utilization did not differ between trials, whereas fat oxidation was elevated during exercise in P. Exercise caused an increase in PGC-1α, and GLUT4 (P < 0.05) independent of exogenous carbohydrate provision. Carbohydrate consumption attenuated the mRNA response in UCP3 (P < 0.05). Conclusions: This study indicates that the provision of exogenous carbohydrate attenuates the stimulation of mRNA expression of UCP3 following exercise in the heat.",

keywords = "Carbohydrate supplementation, Mitochondrial biogenesis, PGC-1α, Substrate utilization, UCP3",

author = "Dumke, {Charles L.} and Slivka, {Dustin R.} and Cuddy, {John S.} and Hailes, {Walter S.} and Ruby, {Brent C.}",

note = "Funding Information: The authors declare that they have no competing interests in access to these data or associations with companies involved with products used in this research. This study was supported by a grant from the Office of Naval Research Grant Award N000140910850. The views and conclusions contained herein are those of the authors and should not be interpreted as necessarily representing the official policies or endorsements, either expressed or implied, of the Office of Naval Research or the U.S. government.",

year = "2013",

month = sep,

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doi = "10.1186/1550-2783-10-40",

language = "English",

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T1 - Skeletal muscle metabolic gene response to carbohydrate feeding during exercise in the heat

AU - Dumke, Charles L.

AU - Slivka, Dustin R.

AU - Cuddy, John S.

AU - Hailes, Walter S.

AU - Ruby, Brent C.

N1 - Funding Information: The authors declare that they have no competing interests in access to these data or associations with companies involved with products used in this research. This study was supported by a grant from the Office of Naval Research Grant Award N000140910850. The views and conclusions contained herein are those of the authors and should not be interpreted as necessarily representing the official policies or endorsements, either expressed or implied, of the Office of Naval Research or the U.S. government.

PY - 2013/9/13

Y1 - 2013/9/13

N2 - Background: Heat stress down-regulates mitochondrial function, while carbohydrate supplementation attenuates the exercise induced stimulation of mitochondrial biogenesis in humans. The effects of exogenous carbohydrate during exercise in the heat on metabolic mRNA have not been investigated in humans. The purpose of this study was to determine the impact of exercise with and without carbohydrate supplementation on skeletal muscle metabolic response in the heat. Methods: Eight recreationally active males (4.05 ± 0.2 L.min-1) completed 2 trials which included 1 hr of cycling at 70% workload max and 3 hr recovery in a hot environment. Both trials were conducted in a climate controlled environmental chamber (38°C and 40% RH). The trials differed by the consumption of either a 6% carbohydrate (CHO) containing beverage (8 ml.kg-1.hr-1) or placebo (P) during exercise in random order. Muscle biopsies were obtained from the vastus lateralis before exercise, immediately post-exercise and at the end of the 3 hr recovery period. Muscle was analyzed for muscle glycogen and mRNA related to metabolic and mitochondrial development (MFN2, PGC-1α, GLUT4, UCP3). Expired gases were measured to determine whole body substrate use during exercise. Results: Carbohydrate oxidation and muscle glycogen utilization did not differ between trials, whereas fat oxidation was elevated during exercise in P. Exercise caused an increase in PGC-1α, and GLUT4 (P < 0.05) independent of exogenous carbohydrate provision. Carbohydrate consumption attenuated the mRNA response in UCP3 (P < 0.05). Conclusions: This study indicates that the provision of exogenous carbohydrate attenuates the stimulation of mRNA expression of UCP3 following exercise in the heat.

AB - Background: Heat stress down-regulates mitochondrial function, while carbohydrate supplementation attenuates the exercise induced stimulation of mitochondrial biogenesis in humans. The effects of exogenous carbohydrate during exercise in the heat on metabolic mRNA have not been investigated in humans. The purpose of this study was to determine the impact of exercise with and without carbohydrate supplementation on skeletal muscle metabolic response in the heat. Methods: Eight recreationally active males (4.05 ± 0.2 L.min-1) completed 2 trials which included 1 hr of cycling at 70% workload max and 3 hr recovery in a hot environment. Both trials were conducted in a climate controlled environmental chamber (38°C and 40% RH). The trials differed by the consumption of either a 6% carbohydrate (CHO) containing beverage (8 ml.kg-1.hr-1) or placebo (P) during exercise in random order. Muscle biopsies were obtained from the vastus lateralis before exercise, immediately post-exercise and at the end of the 3 hr recovery period. Muscle was analyzed for muscle glycogen and mRNA related to metabolic and mitochondrial development (MFN2, PGC-1α, GLUT4, UCP3). Expired gases were measured to determine whole body substrate use during exercise. Results: Carbohydrate oxidation and muscle glycogen utilization did not differ between trials, whereas fat oxidation was elevated during exercise in P. Exercise caused an increase in PGC-1α, and GLUT4 (P < 0.05) independent of exogenous carbohydrate provision. Carbohydrate consumption attenuated the mRNA response in UCP3 (P < 0.05). Conclusions: This study indicates that the provision of exogenous carbohydrate attenuates the stimulation of mRNA expression of UCP3 following exercise in the heat.

KW - Carbohydrate supplementation

KW - Mitochondrial biogenesis

KW - PGC-1α

KW - Substrate utilization

KW - UCP3

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U2 - 10.1186/1550-2783-10-40

DO - 10.1186/1550-2783-10-40

M3 - Article

AN - SCOPUS:84883679712

SN - 1550-2783

VL - 10

JO - Journal of the International Society of Sports Nutrition

JF - Journal of the International Society of Sports Nutrition

M1 - 40

ER -

Skeletal muscle metabolic gene response to carbohydrate feeding during exercise in the heat

Abstract

Funding

Keywords

UN SDGs

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