TY - JOUR
T1 - SNARES and NSF in targeted membrane fusion
AU - Hay, Jesse C.
AU - Scheller, Richard H.
PY - 1997/8
Y1 - 1997/8
N2 - A major current issue in vesicle trafficking is whether NSF (N-ethylmaleimide-sensitive factor) and α-SNAP (α-soluble NSF attachment protein) are required prior to SNARE (SNAP receptor) complex formation to allow vesicle docking, or after docking at a step close to membrane fusion. Recent studies of yeast vacuolar fusion indicated that the requirement for ATP, NSF and α-SNAP could be completely satisfied prior to SNARE docking complex assembly; however, the universality of a predocking role for these factors remains to be established. The vacuolar fusion system has also been used to directly demonstrate a requirement for SNARE proteins on both fusing membranes, verifying a central postulate of current fusion models.
AB - A major current issue in vesicle trafficking is whether NSF (N-ethylmaleimide-sensitive factor) and α-SNAP (α-soluble NSF attachment protein) are required prior to SNARE (SNAP receptor) complex formation to allow vesicle docking, or after docking at a step close to membrane fusion. Recent studies of yeast vacuolar fusion indicated that the requirement for ATP, NSF and α-SNAP could be completely satisfied prior to SNARE docking complex assembly; however, the universality of a predocking role for these factors remains to be established. The vacuolar fusion system has also been used to directly demonstrate a requirement for SNARE proteins on both fusing membranes, verifying a central postulate of current fusion models.
UR - http://www.scopus.com/inward/record.url?scp=0030796026&partnerID=8YFLogxK
U2 - 10.1016/S0955-0674(97)80026-9
DO - 10.1016/S0955-0674(97)80026-9
M3 - Article
C2 - 9261050
AN - SCOPUS:0030796026
SN - 0955-0674
VL - 9
SP - 505
EP - 512
JO - Current Opinion in Cell Biology
JF - Current Opinion in Cell Biology
IS - 4
ER -