Sources of polycyclic aromatic hydrocarbons are associated with gene-specific promoter methylation in women with breast cancer

Alexandra J. White, Jia Chen, Susan L. Teitelbaum, Lauren E. McCullough, Xinran Xu, Yoon Hee Cho, Kathleen Conway, Jan Beyea, Steven D. Stellman, Susan E. Steck, Irina Mordukhovich, Sybil M. Eng, Mary Beth Terry, Lawrence S. Engel, Maureen Hatch, Alfred I. Neugut, Hanina Hibshoosh, Regina M. Santella, Marilie D. Gammon

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Tobacco smoke, diet and indoor/outdoor air pollution, all major sources of polycyclic aromatic hydrocarbons (PAHs), have been associated with breast cancer. Aberrant methylation may be an early event in carcinogenesis, but whether PAHs influence the epigenome is unclear, particularly in breast tissue where methylation may be most relevant. We aimed to evaluate the role of methylation in the association between PAHs and breast cancer. Methods: In a population-based case-control study, we measured promoter methylation of 13 breast cancer-related genes in breast tumor tissue ( n=765-851 cases) and global methylation in peripheral blood (1055 cases/1101 controls). PAH sources (current active smoking, residential environmental tobacco smoke (ETS), vehicular traffic, synthetic log burning, and grilled/smoked meat intake) were evaluated separately. Logistic regression was used to estimate adjusted odds ratios (ORs) and 95% confidence intervals (CIs). Results: When comparing methylated versus unmethylated genes, synthetic log use was associated with increased ORs for CDH1 (OR=2.26, 95%CI=1.06-4.79), HIN1 (OR=2.14, 95%CI=1.34-3.42) and RARβ (OR=1.80, 95%CI=1.16-2.78) and decreased ORs for BRCA1 (OR=0.44, 95%CI=0.30-0.66). Residential ETS was associated with decreased ORs for ESR1 (OR=0.74, 95%CI=0.56-0.99) and CCND2 methylation (OR=0.65, 95%CI=0.44-0.96). Current smoking and vehicular traffic were associated with decreased ORs for DAPK (OR=0.53, 95%CI=0.28-0.99) and increased ORs for TWIST1 methylation (OR=2.79, 95%CI=1.24-6.30), respectively. In controls, synthetic log use was inversely associated with LINE-1 (OR=0.59, 95%CI=0.41-0.86). Discussion: PAH sources were associated with hypo- and hypermethylation at multiple promoter regions in breast tumors and LINE-1 hypomethylation in blood of controls. Methylation may be a potential biologic mechanism for the associations between PAHs and breast cancer incidence.

Original languageEnglish
Pages (from-to)93-100
Number of pages8
JournalEnvironmental Research
Volume145
DOIs
StatePublished - Feb 1 2016

Keywords

  • Air pollution
  • Environmental tobacco smoke
  • Grilled meat
  • Methylation
  • Smoking

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