Structural changes in glycogen phosphorylase induced by phosphorylation

S. R. Sprang; K. R. Acharya; E. J. Goldsmith; D. I. Stuart; K. Varvill; R. J. Fletterick; N. B. Madsen; L. N. Johnson

doi:10.1038/336215a0

Structural changes in glycogen phosphorylase induced by phosphorylation

S. R. Sprang, K. R. Acharya, E. J. Goldsmith, D. I. Stuart, K. Varvill, R. J. Fletterick, N. B. Madsen, L. N. Johnson

Division of Biological and Biomedical Sciences

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281 Scopus citations

Abstract

A comparison of the refined crystal structures of dimeric glycogen phosphorylase b and a reveals structural changes that represent the first step in the activation of the enzyme. On phosphorylation of serine-14, the N-terminus of each subunit assumes an ordered helical conformation and binds to the surface of the dimer. The consequent structural changes at the N- and C-terminal regions lead to strengthened interactions between subunits and alter the binding sites for allosteric effectors and substrates.

Original language	English
Pages (from-to)	215-221
Number of pages	7
Journal	Nature
Volume	336
Issue number	6196
DOIs	https://doi.org/10.1038/336215a0
State	Published - 1988

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title = "Structural changes in glycogen phosphorylase induced by phosphorylation",

abstract = "A comparison of the refined crystal structures of dimeric glycogen phosphorylase b and a reveals structural changes that represent the first step in the activation of the enzyme. On phosphorylation of serine-14, the N-terminus of each subunit assumes an ordered helical conformation and binds to the surface of the dimer. The consequent structural changes at the N- and C-terminal regions lead to strengthened interactions between subunits and alter the binding sites for allosteric effectors and substrates.",

author = "Sprang, \{S. R.\} and Acharya, \{K. R.\} and Goldsmith, \{E. J.\} and Stuart, \{D. I.\} and K. Varvill and Fletterick, \{R. J.\} and Madsen, \{N. B.\} and Johnson, \{L. N.\}",

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doi = "10.1038/336215a0",

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T1 - Structural changes in glycogen phosphorylase induced by phosphorylation

AU - Sprang, S. R.

AU - Acharya, K. R.

AU - Goldsmith, E. J.

AU - Stuart, D. I.

AU - Varvill, K.

AU - Fletterick, R. J.

AU - Madsen, N. B.

AU - Johnson, L. N.

PY - 1988

Y1 - 1988

N2 - A comparison of the refined crystal structures of dimeric glycogen phosphorylase b and a reveals structural changes that represent the first step in the activation of the enzyme. On phosphorylation of serine-14, the N-terminus of each subunit assumes an ordered helical conformation and binds to the surface of the dimer. The consequent structural changes at the N- and C-terminal regions lead to strengthened interactions between subunits and alter the binding sites for allosteric effectors and substrates.

AB - A comparison of the refined crystal structures of dimeric glycogen phosphorylase b and a reveals structural changes that represent the first step in the activation of the enzyme. On phosphorylation of serine-14, the N-terminus of each subunit assumes an ordered helical conformation and binds to the surface of the dimer. The consequent structural changes at the N- and C-terminal regions lead to strengthened interactions between subunits and alter the binding sites for allosteric effectors and substrates.

UR - https://www.scopus.com/pages/publications/0024290703

U2 - 10.1038/336215a0

DO - 10.1038/336215a0

M3 - Article

C2 - 3194008

AN - SCOPUS:0024290703

SN - 0028-0836

VL - 336

SP - 215

EP - 221

JO - Nature

JF - Nature

IS - 6196

ER -

Structural changes in glycogen phosphorylase induced by phosphorylation

Abstract

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